抗肺癌免疫光敏剂的制备及其杀伤作用

The Preparation and Killing Acticity Anti-Lung Cancer Immunophotosensitizer

以碳二亚胺(EDCI)为连接剂将抗肺癌单克隆抗体(McAb 3D3与血卟啉衍生物(HpD)共价连接,制备成免疫光敏剂,经ELISA法测定及溶血试验,结果表明偶联物保留了 McAb的反应性及HpD的光敏活性,偶联物经紫外光谱及荧光光谱扫描分析表明,偶联HpD与非偶联HpD的光谱特性有所差别,体外杀伤试验表明,偶联物的杀伤效率提高了10.5倍,且对非靶细胞的杀伤作用微弱,证明HpD与McAb偶联后,对靶细胞具有特异杀伤作用,可为肿瘤的治疗提供一种新的药物。

In this paper, hematoporphyrin derivative (HpD) was covatently conjugated with monoclonal antibody directed to the lung cancer cell 342. Analyzed with TCL, ELISA and hemolysis of red blood cells, the conjugate was proved that both HpD and McAb retained their original biological activity and specificity, respectively. But the UV absorbance spectra and fluoresence spectra of free HpD and conjugated HpD showed obvious difference. In the presence of light, the conjugate showed 10. 5 fold greater cytotoxicity than free HpD at an equivalent HpD cone, for target cancer cell in vitro, but no cytotoxicity for non-target cancer celt. The result demonstrated that the anti-tumor specificity of the conjugated HpD had been greatly improved and implied that it is possible by using lower dose of drug to minimize undesived side effect. The homing ability of the specific McAb-HpD conjugate may permit a realistic photoimmunotherapy procedure.