摘要
利用高度选育的 8头瘦肉型长白猪为父本 ,16头中国优良地方品种蓝塘猪为母本 ,采用远交系杂交的F2代设计方法 ,建立包括 8头F1公猪、4 0头F1母猪和 2 32头F2 个体的猪资源群体。资源群体的遗传分析表明 ,所测定的 32个性状都有一定的变异 ,主要经济性状的变异系数都在 10 %以上。进一步的方差组分估计表明 ,主要经济性状的加性遗传方差较大。在所测定 6号染色体连锁群的 2 2个微卫星DNA标记中 ,12个表现有多态性 ,全群平均杂合度为 0 5 3,多态信息含量为 0 4 6 ,能较好地为QTL检测提供信息。因此 ,F2 代有较好的分离状态 ,有可能利用该资源群体检测到较多的QTL。
With F_2 design,16 Chinese Lantang sows crossbred with eight highly improved Landrace boars to establish a resource population including 40 F_1 sows,eight F_1 boars,and 232 F_2 pigs.Genetic analysis of the resource population showed that the 32 performance traits displayed some degree of variation,and coefficients of variation of the majority of economic traits exceeded 10%.The variance component analysis revealed that the ratios of additive genetic variance to phenotypic variance of the majority of economic traits were high.Of the 22 microsatellite DNA markers,only 12 microsatellite DNA markers are polymorphisic in this populations.The average heterozygosity of these markers and PIC were 0.53 and 0.46 respectively.The results showed that these markers can provide enough information for QTL mapping.In conclusion,the F_2 pigs were sufficiently segregated,and were capable of serving as a resource population for QTL mapping.
基金
国家重点基础研究发展规划 ( 973计划 )项目 (编号 :G2 0 0 0 0 1610 4)
国家自然科学基金 (编号 :3 980 0 10 3 )
广东省自然科学基金 (编号 :980 15 3 )项目~~
关键词
猪
资源群
F2设计
方差组分
QTL定位
pig
resource population
F_2 design
variance component
QTL mapping
