糖尿病患者眼前房液血管内皮生长因子与转化生长因子β_2的变化

Alterations of VEGF and TGF-β_2 in aqueous humor of diabetic patients

目的:验证糖尿病患者眼前房液中转化生长因子β2(TGF-β2)变化的临床意义,研究TGF-β2在新生血管形成过程中的作用和作用机制及其与血管内皮生长因子(VEGF)之间的关系。方法:收集糖尿病不伴有眼底改变与患有糖尿病视网膜病变白内障患者的前房液标本,并以无糖尿病白内障患者的前房液标本作为对照,用ELISA方法测定了前房液中TGF-β2和VEGF的含量。结果:和对照相比较糖尿病患者眼前房液中VEGF的含量均显著增加,并且随着糖尿病视网膜病变的进展而增高;而糖尿病无眼底改变患者前房液中TGF-β2含量与正常相比无明显变化,单纯型糖尿病视网膜病变患者的TGF-β2含量下降,伴有增生型的视网膜病变患者TGF-β2含量升高。结论:TGF-β2在DR形成过程中有重要作用,在DR后期TGF-β2和VEGF具有协同作用。

AIM: To evaluate the clinical significance of TGF-β2's variation in the aque ous humor of diabetic patients, the role of TGF-β2 in the mechanism of neovascu larization and the relationship between TGF-β2 and VEGF. · METHODS: The aqueous humor specimens of diabetic cataract patients with retin opathy but without eyeground changes were collected, and those of cataract patie nts without diabetes mellitus were selected as the controls. Then the contents o f TGF-β2 and VEGF in the aqueous humor were determined by employing the ELISA t echnique. · RESULTS: Compared with the controls, the content of VEGF in the aqueous humor of diabetic patients increases significantly and grows with the development of diabetic retinopathy. However, there is no significant change of the content of TGF-β2 in the aqueous humor of diabetic patients without eyeground changes in c omparison with the normal. The content of TGF-β2 declines in simple diabetic re tinopathy and increases in proliferative diabetic retinopathy. · CONCLUSION: TGF-β2 plays an important role in the development of diabetic re tinopathy and there is coordination between TGF-β2 and VEGF at the advanced sta ge of diabetic retinopathy. ·