固定化条件对葡萄糖酶电极响应性能的影响

Effect of Immobilization Conditions on the Response of Glucose Enzyme Electrode

用微铂盘电极和交联法制得的酶膜构成葡萄糖酶电极,测定其电流响应特性。研究了酶的固定化条件;酶含量以及载体蛋白和交联剂的用量等,对响应特性的影响,提出较适宜的酶电极制备方法,并检测了所制作的电极在不同pH溶液中的灵敏度,指出其最佳操作条件。

A glucose enzyme electrode was constructed by using a Pt micro-discelectrode covered with a crosslinking enzyme membrane of glucose oxidase (GOD), the current response of the electrode was measured. The effect on the response of enzyme-immobilizing conditions such as the contents of GOD, carrier protein and crosslinking reagent was studied. By comparison, a favorable procedure was suggested for making the enzyme electrode and the sensitivity of the prepared electrode was examined in the test solutions with various pH values to provide the optimum for the electrode operation.