丝裂霉素C磁性纳米微球的制备

Preparation of mitomycin C magnetic nanoparticles

目的:以丝裂霉素C为药物模型,研究磁性纳米抗肿瘤药物的制备工艺及方法。方法:采用正交设计优化工艺并筛选,以纳米级Fe_3O_4为磁性核心,人血清白蛋白为膜材,利用乳液固化法制备包载丝裂霉素C 的磁性纳米微球,并借助透射、扫描电镜观察微球形态,通过激光粒度分析仪作粒度分析,利用高效液相色谱(HPLC)测量载药量及包封率,以磁性测试仪进行体外磁响应性测定。结果:该优化的磁性纳米微球在电镜下呈表面光滑的核壳样球型微粒,平均粒径为217.7nm,微球载药量为7.89%,包封率为90.5%,体外饱和磁化强度为21.85emu·g^(-1)。结论:磁性纳米载药微球为肿瘤的主动靶向治疗提供了一种可能的新剂型,有较好的临床应用前景。

Objective:To prepare anti-tumor magnetic nanoparticles containing mitomycin C(MMC).Methods:Based on an orthogonal test,the formulation and preparation of MMC magneticnanoparticles was optimized.With an emulsion method,the magnetic nanoparticles was formed,usingFe_3O_4 (nanometer grade) as magnetic core and human serum albumin (HSA) as carriers.The nanopar-ticle appearance was examined by both transmission electron microscopy and scanning electron mi-croscopy.The size distribution was determined by a laser light scatting technique.The drug loading andembedding ratio were quantified by HPLC.The magnetization at room temperature was measured by amagnetometer.Results:Under the electron microscopy the optimized magnetic nanoparticles showed asmooth spheroidal particle with core-shell,which had an average diameter value of 217.7 nm.The drugloading and embedding ratio were 7.8 % and 90.5 %,respectively.The saturated magnetization atroom temperature was 21.85emu·g^(-1).Conclusion:The magnetic nanoparticles may offer a novel ap-proach to target the therapeutic basis of tumors.