摘要
目的 评价125Ⅰ-脱氧尿嘧啶核苷(UdR)对膀胱癌细胞Sca-BER生长的辐射生物作用及其影响因素。方法将125Ⅰ UdR加入Sca-BER细胞培养基中,测量细胞摄取125Ⅰ UdR的放射性活度,用细胞克隆形成法测定125Ⅰ UdR 对Sca-BER细胞的生长抑制作用;将125Ⅰ UdR和MTX同时加入Sca-BER细胞培养基中,测量细胞摄取125ⅠUdR的放射性活度,观察MTX协同125Ⅰ UdR的生长抑制作用。结果 培养基中125Ⅰ UdR浓度与Sca-BER细胞摄取量,两者之间呈显著正相关(r=0.9999);Sca-BER细胞存活分数与125IUdR浓度,两者呈负相关(r=-0.9),半数致死剂量(LD50)为(1.17±0.27)kBq／ml。125Ⅰ UdR组细胞存活分数明显低于Na125Ⅰ组;相同浓度125Ⅰ UdR作用于Sca-BER细胞生长过程中,在24 h内细胞摄取量随作用时间的延长而增加,24 h后达最大;甲氨喋呤协同125ⅠUdR抑制Sca-BER细胞生长,使细胞摄取量提高10倍。结论125Ⅰ UdR能掺入到Sca-BER细胞中,并对其有显著辐射抑制效应,说明125Ⅰ UdR可以进一步应用于膀胱癌的放射治疗研究。
Objective To determine the inhibition effects of 125I UdR in human bladder cancer cell line Sca-BER. Methods 125I UdR were added to the RPMI1640 culturing medium. The amount of 125I UdR uptaken by Sca-BER cells was evaluated through measuring the radioactivity per cell; the killing effects of 125I UdR on Sca-BER cells were estimated by colony forming method. To determine the co-inhibition effects of MTX, 125I UdR and MTX were added tomedium, measuring the radioactivity per cell. Results The amounts of 125I UdR uptaken by Sca-BER was growing up as function of 125I UdR dose in the medium (r=0.99) . The surviving fraction was correlated with the concentration of 125I UdR (r=-0.9). The LD50 of 125I UdR group was (1.17±0.27) kBq/ml and the surviving fraction of 125I UdR group was significantly lower than that of Na125I group(P<0.001) .The 125I UdR of the same concentration was added to the medium. With time elapsing, the amount of 125I UdR uptaken was inereased. At the 24th hour of addition of 125I UdR incorporation got to the peak. 125I UdR and MTX were added to medium, the amount of I25I UdR uptaken was inereased for 10 times. Conclusions 125I UdR can be incorporated into Sca-BER cells, and the cocen-tration of 125I UdR in Sca-BER cells was influenced by I25I UdR dose in the medium. The inhibitory radiation effects of L25I UdR on Sca-BER cells are obvious.
出处
《苏州大学学报（医学版）》
CAS
2004年第6期794-797,817,共5页
Suzhou University Journal of Medical Science
基金
江苏省135重点人才资助项目(122003094)
