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rhIL-24刺激PBMC产生IFN-γ、IL-6的初步研究 被引量：2

Study on Effects of rhIL-24 on the Production of IFN-γ, IL-6 by Using PBMC
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摘要 目的 研究重组人白细胞介素24(rhlL-24)对外周血单个核细胞产生γ干扰素(IFN-γ)、白细胞介素6(IL-6)的刺激效应。方法 正常人的外周血单个核细胞(PBMC)经含有rhlL-24的CHO细胞表达上清刺激48 h、72 h,取其刺激PBMC培养上清液用酶联免疫吸附试验((ELISA)法测定IFN-γ和IL-6含量。结果 加含rhlL-24的CHO细胞表达上清的实验组上清中IFN-γ和IL-6含量明显高于对照组(P<0.01)。结论 rhlL-24对外周血单个核细胞分泌IFN-γ和IL-6具有刺激效应,说明rhlL-24具有明显的免疫调节功能。导师。 Objective To evaluate the effect of rhIL-24 stimulating on peripheral blood mononuclear cells (PMBC) to produce IFN-γand IL-6. Methods Normal PBMCs were cultured with the expression supernatant of the rhIL-24 CHO cell by 48 h and 72 h, its stimulated product was detected by ELISA. Results The quantity of IFN-7 and IL-6 in the groups of cells cultured with the expression supernatant of the rhIL-24 CHO cell was obvious higher than that in the groups without IFN-γand IL-6 (P<0.01). Conclusion rhIL-24 can induce PBMC to secrete IFN-γ and IL-6, Elucidation rhIL-24 have obvious function to regulate immunity.
作者 叶建新 陈雄艳 盛伟华 丁云芳 谢宇峰 缪竞诚 杨吉成
机构地区 苏州大学附属儿童医院 苏州大学医学院 细胞和分子生物学教研室
出处 《苏州大学学报（医学版）》 CAS 2004年第6期813-814,共2页 Suzhou University Journal of Medical Science
关键词 重组人白细胞介素24 外周血单个核细胞 Γ干扰素 白细胞介素6 rhIL-24 PBMC IFN-7 IL-6
分类号 R392.1 [医药卫生—免疫学]
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参考文献7

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二级参考文献7

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共引文献7

同被引文献21

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引证文献2

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苏州大学学报（医学版）
2004年 第6期

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