慢性肺疾病早产鼠BALF及肺组织中脂质过氧化的同步研究

Researches on lipid peroxidation of bronchoalveolar lavage and lung in premature rat with chronic lung disease

目的 为探讨高氧致早产儿慢性肺疾病 (CLD)发生中支气管肺泡灌洗液 (BALF)及肺组织中脂质过氧化的变化规律及相互间关系。方法 采用高浓度氧致早产鼠CLD模型为研究对象 ,应用分光光度计比色法同步测定BALF和肺组织超氧化物岐化酶 (SOD)活性及脂质过氧化产物丙二醛 (MDA)的含量。结果 两组的BALF及肺组织中SOD活性均无差异(P >0 .0 5 ) ;而实验组 ,BALF及肺组织中MDA的水平呈同相变化 ,即吸高氧 3d明显升高 (P <0 .0 5 ) ,7d达高峰 (P <0 .0 1) ,持续 1w后逐渐下降 ,2 1d仍高于正常水平 (P <0 .0 5 )。结论 肺组织的氧自由基损伤贯穿高氧致CLD发生、发展全过程中 ;BALF中MDA水平可间接反应肺组织的氧自由基损伤程度。

Objective: To investigate dynamic changes of lipid peroxidation of bronchoalveolar lavage and lung in hyperoxia-induced chronic lung disease (CLD). Methods: Malondialdehyde (MDA) concentration and superoxide dismutase (SOD) activity from lung specimen of day 1,3,7,14 and 21 in 80 premature rat with hyperoxia-induced CLD and 80 control premature rats were assayed with double wavelength scanning by spectrophotometer. Results: SOD activity was no significant difference among any groups. MDA level was increased in day3, and peak level was in day 7, and then was decreased gradually but still maintained at higher level than control group at day 21. Conclusions: Injury of oxygenic free redical in lung companies with the process of hyperoxia-induced CLD; The level of MDA reacts indirect lung injuy.