蝉拟青霉对大鼠营养状况的影响

Effect of Paecilomyces cicadae on the nutritious condition of rats

目的 :观察蝉拟青霉菌丝体水提取物对正常及环磷酰胺所致免疫抑制大鼠血液蛋白质、白细胞水平及肝细胞形态结构的影响 ,探讨其对机体营养状况的影响。方法 :用HiCN法、双缩脲法、溴甲酚绿法分别测定血红蛋白、血清总蛋白、白蛋白含量 ;测定外周血白细胞数 ;并在电子显微镜下观察肝细胞的形态结构。结果 :蝉拟青霉使正常大鼠血清总蛋白、球蛋白含量显著增加 (P <0 .0 1 ) ,并能拮抗由于使用环磷酰胺所造成的血红蛋白、血清总蛋白、球蛋白含量的降低 (P <0 .0 5 ) ;使正常大鼠血白细胞数明显升高 (P <0 .0 5 ) ,并能阻遏由于使用环磷酰胺所致的白细胞降低 (P <0 .0 5 ) ;使正常大鼠肝细胞粗面内质网、线粒体增多。结论 :蝉拟青霉对机体的营养状况有改善作用。

Objective: To investigate the change of the level of protiens and the number of white blood cell (WBC) in blood, and the shape and structure of liver cell in normal and immunosuppressed rats caused by cyclophosphamide (Cy) after subcutaneous injection of water extract from the culture mycelium of Paecilomyces cicadae (P. Cicadae), and to explore the effect of P. Cicadae on the nutritious condition of rats. Methods:The hemoglobin level in the red blood cells and the contents of the total proteins and albumin in the serum were determined by HiCN method, biuret reaction and bromocresol green method respectively, and the number of WBC in peripheral blood also be measured, and the shape and structure of liver cell were observed under electron microscope.Results:After administering P. cicadae to rats, the following results were observed: The level of total proteins and globulin in the serum in normal rats were notably increased(P<0.01), and the decrease of the contents of hemoglobin and the total proteins and globulin in the blood in Cy-immunosuppressed rats were restored(P<0.05). Not only the number of WBC in peripheral blood in normal rats was significantly elevated(P<0.05), but also the decrease of WBC in Cy-immunosuppressed rats was inhibited obviously(P<0.05). The number of rough endoplasmic reticulum(RER) and mitochondrion in liver cell increased evidently in normal rats under electron microscope.Conclusion:P. cicadae can improve the nutritious condition of rats.