CVIC/C复合材料及其表面HA涂层成骨细胞体外响应行为对比研究

Response of Osteoblast to CVI C/C and HA Coating on CVI C/C in Vitro

采用声电化学工艺在 CVI C/C 复合材料表面制备了 HA 涂层,分别用 SEM,XPS,EDAX 和 XRD 表征了 CVIC/C 和 HA 的表面形貌、组成和结构,同时对比了成骨细胞在其表面的粘附、增殖和碱性磷酸酶活性的表达。制备的HA 涂层为球状形貌,由纳米片状晶体构成。细胞培养初期,粘附于 HA 涂层表面的细胞数与 CVI C/C 表面的细胞数无显著差别,成骨细胞在 CVI C/C 表面易铺展在孔的边缘,8 h 后粘附于 HA 表面的成骨细胞数显著多于 CVI C/C 表面。HA 涂层提高了细胞的增殖能力,成骨细胞碱性磷酸酶在 HA 涂层表面的表达也优于 CVI C/C 材料。结果表明,声电化学制备的 HA 涂层提高了 CVI C/C 材料的生物活性和生物相容性,可以加速成骨细胞的粘附、增殖、分化和成熟。成骨细胞在材料表面的粘附机制是由多因素综合作用的,材料的表面形貌和生物活性在细胞粘附过程的早期发挥着重要作用。

Hydroxyapatite (HA) coating was deposited on chemical vapor infiltration carbon/carbon composite (CVI C/C) by catholic sonoelectrodeposition technique. The morphologies and structure of HA and CVI C/C were characterized by SEM, XPS, EDAX and XRD. In order to investigate the bioactivity and biocompatibility of HA coating, CVI C/C, MC-3T3-E1 osteoblast-like cells were cultured on the surface of two materials in vitro and attachment experiment, proliferation experiment and alkaline phosphatase (ALP) activity experiment were done. The results showed that the HA coating with spherical morphology was composed of flake-like nanocrystal. No statistic difference was found at the primary period of cell attachment experiment. But after 8 hours culture, the number of osteoblasts adhered to HA coating were significantly more than that of adhered to CVI C/C. The SEM observation revealed that osteoblasts spread well on both of materials. It was also found that HA coating enhanced cell proliferation and ALP expression. So it demonstrated that HA coating deposited by catholic sonoelectrodeposition processing improved the biocompatibility of CVI C/C and possessed good bioactivity, and HA coating could speed up the attachment, proliferation, differentiation and maturation of osteoblasts. The attachment mechanism of osteoblasts was a complex course, which was influenced by many factors. The morphology and the bioactivity of material's surface played important roles at the initial period of cell attachment.