阴沟肠杆菌Ⅰ类整合酶基因及质粒AmpC酶基因检测

Detection of the class 1 integrase gene and plasmid-AmpC enzyme genes in Enterobacter cloacae

目的明确我院临床分离的阴沟肠杆菌中Ⅰ类整合酶基因(intⅠ1)、qacE△1-sulⅠ基因和质粒Am鄄pC酶基因(AmpCMIR、AmpCDHA)存在状况。方法采用ATB药敏试验板微量肉汤法测定临床分离的20株阴沟肠杆菌对20种抗菌药物的敏感性,采用聚合酶链反应(PCR)技术检测耐药基因。结果该20株菌呈现多重耐药,对亚胺培南和美罗培南均敏感,对阿莫西林、阿莫西林/克拉维酸、头孢噻吩和头孢西丁完全耐药,头孢吡肟和复方新诺明的耐药率分别为25.0%和85.0%,对氨基糖苷类抗生素的耐药率在60.0%～90.0%之间,其余的耐药率在80.0%～95.0%之间。intⅠ1、qacE△1-sulⅠ、MIR和DHA基因的阳性株数(%)分别为19株(95.0%)、17株(85.0%)、17株(85.0%)、1株(5.0%)。结论我院临床分离的阴沟肠杆菌多重耐药严重,intⅠ1、qacE△1-sulⅠ和MIR基因携带率很高。在阴沟肠杆菌中检出intⅠ1、qacE△1-sulⅠ以及质粒型AmpCMIR基因在我国大陆均属首次报道。

Objective To investigate the genes of the class 1 integrase(intⅠ1), qacE△1-sulⅠ, and plasmid-AmpC enzyme(AmpC MIR and AmpC DHA) in Enterobacter cloacae. Methods 20 strains of Enterobacter cloacae were isolated from the sections of in-patients, and their susceptibilities of 20 kinds of antimicrobial agents against these strains by microdilute tests. Genotypes was analyzed by polymerase chain reaction (PCR). Results 20 strains of Enterobacter cloacae showed multiple-drug resistance. There is no strain resistant to Imipenam and Meropenem. The resistant rates to Cefepime and Cotrimoxazol were 25.0% and 85.0% respectively, and with 60.0% to 100.0% of other antimicrobial agents. The positive rates of intⅠ1, qacE△1-sulⅠ, MIR, and DHA genes were 95.0%,85.0%,85.0%, and 5.0% respectively. Conclusions There are very high positive rate of intⅠ1,qacE△1-sulⅠ, and plasmid-AmpC MIR genes in Enterobacter cloacae isolated from the sections of in-patients in our hospital. The genes of intⅠ1, qacE△1-sulⅠ, and plasmid-AmpC MIR in Enterobacter cloacae are firstly reported in P.R.C.