赤眼蜂寄生对亚洲玉米螟卵内蛋白质变化的影响

A Primary Study on the Influence of Parasitization by Trichogramma ostriniae on Protein Changes in the Eggs of Ostrinia furnacalis

采用SDS聚丙烯酰胺凝胶电泳方法比较了玉米螟赤眼蜂寄生对亚洲玉米螟卵内蛋白质变化的影响。结果发现,寄生发生48h后,蛋白质谱带出现明显差异。被寄生和未被寄生卵内含有的97kD蛋白质在60h后明显减少,而前者含有的90kD蛋白质在寄生发生60h时含量明显增加;此外,被寄生和未被寄生卵电泳后均出现约50和44kD蛋白质条带,但在寄生发生84h后,这两条蛋白质带明显变浅,且未被寄生卵的改变更明显。同一时期被寄生和未被寄生卵间约有50%蛋白质条带出现在相同位置,而72、96h的寄生和未被寄生卵间约有30%。被寄生和未被寄生卵之间,同一时期的蛋白质条带数一般相差4条左右,但寄生发生6h后相差的条带数多达9条,这一时期出现与寄生和抗寄生有关的特异性蛋白质的可能性较大。

Protein changes in the eggs of Ostrinia furnacalis parasitized by egg parasitoid, Trichogramma ostriniae, were studied by SDS-PAGE. The results showed that the changes took place 48 h after parasitization. The quantity of 97 kD protein both in parasitized and unparasitized eggs decreased significantly after 60 h. The 90 kD protein appeared in the parasitized eggs at 48 h and its quantity increased at 60 h. The quantity of 50 and 44 kD proteins both in parasitized and unparasitized eggs decreased after 84 h, in particular in unparasitized eggs. The number of protein bands that migrated to the similar distances in parasitized and unparasitized eggs with similar development time was about half of the total number of protein bands in parasitized and unparasitized eggs at any developmental stages, except for 72 h and 96 h. There were about 4 fewer or more protein bands in parasitized eggs than in unparasitized eggs at any developmental stages except for 6 h, when there were 9 more protein bands in parasitized eggs than in unparasitized eggs. The special protein responsible for defence and anti-defense during parasitization maybe occur at this stage.