玫烟色拟青霉分离株的生物学特性及其对烟粉虱的致病力

Biology of Paecilomyces fumosa-roseus Isolates and Their Pathogenicity Against Bemisia tabaci

对广州地区玫烟色拟青霉16个分离株的菌落生长速率、产孢量、分生孢子萌发率,及其对烟粉虱2龄若虫的致病性进行了比较研究。结果表明:不同分离株的菌落生长速率差异不显著,但产孢量、孢子萌发率和对烟粉虱2龄的致病力差异显著。经过综合比较,筛选出PF01 N4和PF01 N8两个优良分离株,它们具有产孢量大(2 8×109和2 95×109个孢子/ml)、孢子萌发率高(89 06%和87 7%)、致病力强(7d后对烟粉虱2龄若虫的致病力分别为99 22%和97 79%)等特点。

Paecilomyces fumosa-roseus is one of the most important entomopathogens of whitefly. The preliminary study results showed that significant differences exist in the sporulation yields, germination rate and pathogenicity against the second instar nymph of Bemisia tabaci among sixteen Paecilomyces fumosa-roseus isolates. But in the mycelial growth there was no obvious difference. By comprehensive comparison of sixteen isolates, the PF01-N4 and PF01-N8 dominant isolates were selected because of high pathogenicity (99.22% and 97.79% mortality against the second instar nymph of Bemisia tabaci respectively), high germination rate of conidia (89.06% and 87.7% respectively) and more sporulation yield (2.8×10~9 and 2.95×10~9 spore/ml respectively). The laboratory study results are promising the potential of P. fumosa-roseus as a microbial control agent against B. tabaci.