环孢素A体外抗AF18标记的曼氏血吸虫童虫的研究(英文)

In Vitro Effect of Cyclosporine A on Juvenile Schistosoma mansoni Labeled by AF18 (5-N-octadecanoyl aminofluorescin)

目的 探讨环孢素A体外抗AF18标记的曼氏血吸虫童虫的作用以及虫体表膜流动性。 方法 制备童虫 ,环孢素A体外作用 ,AF18标记 ,荧光显微镜观察。 结果 童虫损伤或死亡 ,虫体逐渐从绿色变成黄色 ,虫体表面损伤。 结论 环孢素A增加童虫AF18的含量 ,减低童虫表膜流动性。

Objective To explore the in vitro effect of cyclosporine A on the tegument of juvenile Schistosoma mansoni labeled by AF18 and investigate the effect of cyclosporine A on schistosomula surface membrane fluidity. Methods Preparation of transformed schistosomula, adding cyclosporine A into tubes containing schistosomula and labeling of transformed schistosomula with AF18,then observe schistosomula under fluorescence microscope. Results Schistosomula of different groups labeled by AF 18 were damaged by cyclosporine A in vitro. Conclusion Cyclosporine A increases the uptake of AF18 by schistsomula in vitro which is dose-dependent, and decreases the parasite surface membrane fluidity.