枸橼酸钠抗凝剂对疟原虫生长活性的影响(英文)

Effect of Sodium Citrate Based Anticoagulants on the Growth Activity of Malaria Parasites

目的 了解枸橼酸钠抗凝剂对疟原虫生长活性的影响。 方法 将疟原虫经抗凝剂 (ACD ,CD和SC)处理后以感染率为指标检测抗凝剂对虫体的影响。未同步化的恶性疟原虫分别使用 3种不同浓度抗凝剂于 3 7℃作用 3h。处理后的红细胞与正常虫体混合 ,同时处理后的虫体与正常红细胞混合 ,随后观察两种培养混合物的感染率以确定抗凝剂作用的靶细胞。同上处理期同步化的疟原虫 (环状体 ,滋养体和裂殖体 )以观察抗凝剂作用的期特异性。将伯氏疟原虫用抗凝剂处理后接种小鼠 ,通过感染率的变化观察抗凝剂对鼠疟的影响。 结果 3种抗凝剂均可抑制疟原虫的生长 ,其中ACD影响最甚。以抗凝剂分别处理红细胞和疟原虫 ,结果表明抗凝剂作用于虫体而非红细胞。处理同步化的虫体表明抗凝剂对裂殖体的抑制最为显著。同样处理伯氏疟原虫后接种小鼠进一步验证了抗凝剂对恶性疟原虫作用的抑制性效应。 结论 ACD对疟原虫的抑制性效应最为明显 ,SC是疟原虫试验中枸橼酸钠抗凝剂的首选。

Objective To study the effect of anticoagulants based on sodium citrate on the growth activity of malaria parasites. Methods The parasites were treated with 3 anticoagulants (ACD, CD and SC), respectively, and the parasitemia was determined to measure the effect of the anticoagulants on the growth of the parasites. Unsynchronized Plasmodium falciparum was treated with the anticoagulants at different concentrations for 3 h at 37 ℃. Treated erythrocytes were mixed with normal parasites or treated parasites with normal erythrocytes, which was followed by parasitemia determination of the two cultures to determine the cell target of the anticoagulants. Stage-synchronized parasites (ring, trophozoite and schizont) were treated as above to investigate the stage target. P.berghei was also treated with anticoagulants and inoculated in mice to detect the effect of anticoagulants on the animal malaria parasite by counting the parasitemia. Results all 3 anticoagulants inhibited falciparum parasite growth and ACD had the strongest potency. The treatments of the erythrocyte and the parasite with anticoagulants respectively showed that the anticoagulants targeted the parasites rather than normal erythrocytes. Stage-synchronized parasite treatment suggested anticoagulants primarily inhibited schizonts. The effect of anticoagulants on P.berghei was similar to that on P.falciparum. Conclusion ACD showed the most significant inhibitive effect on the growth of malaria parasites and SC was the best anticoagulant based on sodium citrate for malarial experiments.