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莫比可对糖尿病肾病大鼠肾脏的保护作用 被引量：4

Mechanism and effect of cyclooxygenase-2 inhibitor meloxicam on the protection of diabetic nephropathy in rats
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摘要 目的 :探讨莫比可对糖尿病肾病 (DN)大鼠肾脏的保护作用。方法 :将链脲佐菌素所诱导的SD清洁级DN大鼠 2 8只随机分为糖尿病肾病未干预组和糖尿病肾病莫比可干预组 ,每组各 14只。另将 12只鼠龄及体重相等的SD大鼠作为正常对照组 ,糖尿病肾病模型成功后开始给予药物干预 ,药物干预 6周后 ,收集 2 4h尿液及血标本 ,处死大鼠后 ,取肾组织行免疫组织化学检查。结果 :糖尿病肾病大鼠肾组织COX 2表达明显增加 ,尿蛋白增加 ,肌酐清除率上升 ,莫比可使尿蛋白减少 (P <0 .0 1) ,肌酐清除率降低 (P <0 .0 1) ,COX 2表达明显下降。结论 :选择性COX 2抑制剂莫比可通过部分抑制COX 2的活性而起到保护肾脏的作用 ,其机制可能与影响COX Objective To investigate the renal protective effect of specfic cyclooxygenase 2 inhibitor meloxicam on the renal of diabetic nephropathy rats.Methods Twenty-eight Sprague-Dawley (SD) rats with diabetic nephropathy by intraperitoneal injection of streptozotocin were randomly divided into 2 groups: diabetic nephropathy without any treatment (Group D,n=14), and diabetic nephropathy treated with meloxicam.(Group M,n=14).Another 12 normal rats were divided into normal control (Group N, n=12), and then Group M began to receive a blocker of cyclooxygenase 2 inhibitor, meloxicam [2 mg/(kg·d)].Six weeks after the induction of DN, 24 h urine and blood samples were collected and kept at -70℃.Then rats were killed.Their kidneys were removed and longitudinal halved.One half was placed and stored in fixation solution until COX-2 immunohistochemistry was determined. Results The creatinine clearance rate,uria protein, and the expression of COX-2 in renal and urinary TXB 2 in diabetic rats were significantly higher than in Group N.Meloxicam decreased uria protein or creatinine clearance rate significantly(P<0.01). Immunohistochemical analysis revealed the decreased expression of COX-2 in the renal of Group M by the meloxicam treatment. Conclusion Meloxicam can protect diabetic kidney,somehow through deceasing the renal expression of COX-2.
作者 刘志纯 周巧玲 欧阳春 邓声莉
机构地区 中南大学湘雅医院肾内科
出处 《中南大学学报（医学版）》 CAS CSCD 北大核心 2004年第6期635-638,共4页 Journal of Central South University ：Medical Science
关键词 莫比可 糖尿病肾病 COX-2表达 保护作用 大鼠肾脏 药物干预 肌酐清除率 清洁级 SD大鼠 链脲佐菌素 diabetic nephropathy COX-2 meloxicam
分类号 R587.1 [医药卫生—内分泌]
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参考文献8

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共引文献314

同被引文献28

  • 1陈芳,孙世澜,孙汉英.环氧化酶-2抑制剂对糖尿病大鼠肾脏活性作用的研究[J].中国血液流变学杂志,2005,15(2):185-190. 被引量：7
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引证文献4

二级引证文献34

中南大学学报（医学版）
2004年 第6期

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