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沙眼衣原体感染Hela229细胞诱导SOCS-1,3的表达 被引量：4

Expression of SOCS-1, 3 induced by Chlamydia trachomatis-infected Hela229
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摘要 目的 :探讨SOCS的表达与沙眼衣原体抑制宿主免疫功能导致持续性感染之间的关系。方法 :应用RT PCR和Westernblotting检测沙眼衣原体感染和未感染Hela2 2 9细胞SOCS 1,3mRNA水平和蛋白质水平的表达 ;免疫荧光检测反义寡核苷酸阻断SOCS 1,3表达后对沙眼衣原体增殖的影响。结果 :沙眼衣原体感染Hela2 2 9细胞后可诱导SOCS 1,3的表达 ,阻断SOCS 1,3表达后沙眼衣原体增殖受到抑制。结论 :沙眼衣原体感染Hela2 2 9细胞后可诱导SOCS 1,3的表达 ,SOCS 1,3的表达是沙眼衣原体抑制宿主免疫功能 。 Objective To explore the relationship between SOCS, immune suppression and chlamydial persistence of Chlamydia trachomatis-infected cells. Methods After being infected with Chlamydia trachomatis, RT-PCR was used to examine the expression of SOCS-1,3 mRNA and Western blotting to examine the expression of SOCS-1,3 protein in Hela229 cells. Immunofluorescence was used to examine the effect of SOCS obstruction on the multiplication of Chlamydia trachomatis. Results Chlamydia trachomatis-infected Hela229 cells can induce the expression of SOCS-1,3 both on mRNA level and on protein level. The multiplicity of Chlamydia trchomatis can be suppressed after the expression of SOCS-1,3 was obstructed. Conclusion Chlamydia trachomatis-infected Hela229 cells can induce the expression of SOCS-1,3, which is one of the mechanisms that Chlamydia trachomatis can suppress host immune and result in persistence.
作者 魏秀青 程文 严杰 梅冰 霍治 余平
机构地区 中南大学湘雅医学院免疫学教研室
出处 《中南大学学报（医学版）》 CAS CSCD 北大核心 2004年第6期639-642,共4页 Journal of Central South University ：Medical Science
基金 湖南省科技厅项目 (0 2ssy30 1 0 9)
关键词 SOCS 表达 沙眼衣原体感染 细胞诱导 阻断 增殖 抑制 MRNA水平 免疫荧光检测 RT—PCR Chlamydia trachomatis Hela229 SOCS-1, 3
分类号 R374.1 [医药卫生—病原生物学]
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参考文献15

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共引文献29

同被引文献46

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引证文献4

二级引证文献5

中南大学学报（医学版）
2004年 第6期

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