幽门螺杆菌感染与胃癌侵袭转移及MMP-2,TIMP-2表达的关系

Helicobacter pylori infection,the invasion and metastasis of the gastric carcinoma,and the expression of MMP-2 and TIMP-2

目的 :探讨H .pylori感染与胃癌侵袭转移及MMP 2 ,TIMP 2表达的关系。方法 :采用Warthin starry组织染色法、快速尿素酶试验及14 C UBT检测H .pylori感染 ,免疫组织化学法检测 2 2例H .pylori感染胃癌与 2 2例无H .pylori感染胃癌组织MMP 2 ,TIMP 2表达。结果 :H .pylori感染胃癌组浆膜侵袭率与淋巴结转移率分别为81.8%与 72 .7% ,高于无H .pylori感染胃癌组 (P <0 .0 5 )。胃癌浆膜侵袭组、淋巴结转移组MMP 2表达阳性率分别为 96 .6 %与 10 0 .0 % ,高于无浆膜侵袭组、无淋巴结转移组 (分别为 4 0 .0 %与 5 0 .0 % ) ;TIMP 2表达阳性率分别为4 8.3%与 4 5 .8% ,低于无浆膜侵袭组、无淋巴结转移组 (分别为 86 .7%与 80 .0 % ) ;MMP 2 /TIMP 2值分别为 6 .86与 7.94 ,高于无浆膜侵袭组、无淋巴结转移组 (分别为 0 .87与 1.0 7)。H .pylori感染胃癌组MMP 2表达阳性率为90 .9% ,高于无H .pylori感染胃癌组 (6 3.6 % ) ;TIMP 2表达阳性率为 36 .4 % ,低于无H .pylori感染胃癌组 (P <0 .0 1) ;MMP 2 /TIMP 2值为 7.73,高于无H .pylori感染胃癌组 (P <0 .0 1)。结论 :H .pylori感染胃癌浆膜侵袭和淋巴结转移增多 ,与MMP 2表达增加、TIMP 2表达减少、MMP 2 /TIMP

Objective To explore the relationship among Helicobacter pylori (H.pylori) infection, the invasion and metastasis of the gastric carcinoma, and the MMP-2 and TIMP-2 expression.Methods The Warthin-starry method, rapid urease test and 14C-UBT were used to detect the infection of H.pylori.The expression of MMP-2 and TIMP-2 in 22 patients with gastric carcinoma infected by H.pylori and 22 patients with gastric carcinoma but not infected were detected by immunohistochemistry method. Results The incidence of serosal invasion and lymph node metastasis in patients with gastric carcinoma infected by H.pylori ( 81.8% and 72.7% respectively) was significantly higher than that in patients with gastric carcinoma but not infected ( 50.0% and 36.4% respectively, P<0.05 ).The positive rates of MMP-2 expression in the invasive group (96.6%) and in the metastasis group (100.0%) were significantly higher than that in the non-invasive group (40.0%) and the non-metastasis group (50.0%), whereas the positive rates of TIMP-2 expression in the invasive group (48.3%) and in the metastasis group (45.8%) were significantly lower than that in the non- invasive group (86.7%) and the non-metastasis group ( 80.0%).The ratios of MMP-2/TIMP-2 in the invasive group (6.86) and in the metastasis group ( 7.94) were significantly higher than that in the non-invasive group (0.87) and the non-metastasis group (1.07).The positive rate of MMP-2 expression in patients with gastric carcinoma infected by H.pylori (90.9%) was significantly higher than that in patients with gastric carcinoma but not infected (63.6%),whereas the positive rate of TIMP-2 expression in patients with gastric carcinoma infected by H.pylori ( 36.4%) was significantly lower than that in patients with gastric carcinoma but not infected(P<0.01).The ratio of MMP-2/TIMP-2 in patients with gastric carcinoma infected by H.pylori (7.73) was significantly higher than that in patients with gastric carcinoma not infected (P<0.01). Conclusion It is associated with the increase of the MMP-2 expression, the decrease of the TIMP-2 expression and the increase of the ratio of MMP-2/TIMP-2 that the incidence of serosal invasion and lymph node metastasis in patients with gastric carcinoma increases by H.pylori infection.