戊巴比妥和异丙酚制作大鼠局灶性脑缺血模型的对比研究

Comparison of pentobarbital and propofol on the outcome of focal cerebral ischemia model in rats

目的 :比较戊巴比妥和异丙酚对大鼠局灶性脑缺血模型病理损伤的影响 ,评价异丙酚在大鼠局灶性脑缺血模型制作中的应用。方法 :将 30只雄性SD大鼠在戊巴比妥或异丙酚腹腔麻醉下制作成永久性大脑中动脉阻塞模型 (n =15 )。术后 4h参照改良的Bederson′s评分方法进行神经功能缺损评分 ,2 4h采用TTC染色确定脑梗死体积 ,3d行TUNEL和甲苯胺蓝染色分别测定半暗带内的凋亡细胞和存活神经元密度。结果 :神经功能缺损评分(1.4 6± 0 .98vs1.2 9± 0 .72 ) ,梗死体积 [(37.8± 4 .95 ) %vs(31.1± 5 .0 9) % ]和半暗带内神经元密度 [(74 0± 2 4 )个/mm2 vs (794± 2 3)个 /mm2 ]在戊巴比妥组和异丙酚组间差异无统计学意义 (P >0 .0 5 ) ,但异丙酚组半暗带内凋亡细胞的密度高于戊巴比妥组 [(35 6± 2 0 )个 /mm2 vs (2 6 2± 17)个 /mm2 ,P <0 .0 5 ]。结论 :异丙酚麻醉下制作大鼠局灶性脑缺血模型可获得与戊巴比妥相似的神经功能缺损评分、梗死体积和半暗带内存活神经元数量 ,但异丙酚能促进半暗带内的细胞凋亡。在评价一些药物或方法对局灶性脑缺血后细胞凋亡的影响时 。

Objective To compare the effects of pentobarbital and propofol on the outcome of focal cerebral ischemia model, and to evaluate the availability of propofol in setting the focal cerebral ischemia. Methods Thirty male Sprague-Dawley rats were subjected to middle cerebral artery occlusion (MCAO) under pentobarbital or propofol intraperitoneal anesthesia (n=15 each). A modified Bederson′s scoring system was adopted to assess neurological scoring at 4 h after the MCAO. TTC staining was used to determine the infarct size at 24 h after the MCAO. At day 3 after the MCAO, TUNEL and toluidine blue staining were performed to measure the density of apoptotic cells and surviving neurons in the penumbra. Results Neither neurological scoring (1.46±0.98 vs 1.29±0.72), infarct size [(37.8±4.95)% vs (31.1± 5.09)% ] nor neuronal density in the penumbra [(740±24) neurons/mm 2 vs (794±23) neurons/mm 2] was statistically different between pentobarbital and propofol groups (P>0.05). But apoptotic cell density in the penumbra was significantly higher in the propofol group than that in the pentobarbital group [(356±20) cells/mm 2 vs ( 262±17) cells/mm 2, P<0.05].Conclusion In the establishment of the focal ischemia model in rats, anesthesia with propofol or pentobarbital induces similar neurological scoring, infarct size and survived neurons in the penumbra. Propofol anesthesia increases cell apoptosis in the penumbra compared with pentobarbital and its application might be an unsuitable anesthetic method in the model for evaluating the effect of procedures or drugs on cell apoptosis.