摘要
对油葵子叶外植体在含有不同激素浓度的培养基上进行了诱导、分化及成苗的研究,建立起一套完整的再生体系。在MS添加IAA1mg/L和6-BA4mg/L的培养基上诱导的愈伤质量较好,在MS添加IAA0.01mg/L和6-BA0.04mg/L的分化培养基上能够实现较高的不定芽再生。基因型间再生能力差异明显,8份材料中以康地6R为最佳。不定芽在1/2MS培养基上能健康成苗。
The factors that influence callus induction, shoot differentiation and growth of Helianthus annuus cotyledon were studied and an efficient regeneration system was established . The results showed that the callus were preferably induced from the medium of MS+1mg/L IAA+4mg/L 6-BA,and shoot regeneration were better achieved from the medium of MS+0.01mg/L IAA+0.04mg/L 6-BA.The regeneration ability of different genotypes was obviously different, among which Kangdi 6R was the best . The shoots could grow up well from the medium of 1/2MS without addition of hormone.
出处
《中国油料作物学报》
CAS
CSCD
北大核心
2004年第4期16-19,共4页
Chinese Journal of Oil Crop Sciences
关键词
向日葵
子叶
芽分化
Helianthus annuus L.
Cotyledon
Shoot differentiation
