摘要
目的 :观察以胶原缓释rhBMP - 2复合BMSCs及珊瑚构建的组织工程骨异位成骨的能力。方法 :构建 3种复合支架材料 :1)rhBMP - 2 /珊瑚 ;2 )胶原 /rhBMP - 2 /珊瑚 ;3)BMSCs/胶原 /rhBMP - 2 /珊瑚。分别植入裸鼠皮下 ,8周后观察成骨情况 ,并作比较。结果 :第 3组材料异位成骨的能力最强 ,第 2组次之 ,第 1组较弱。结论 :动物实验中胶原是rhBMP - 2适宜的缓释载体 。
Objective: To evaluate ectopia osteogenesis by tissue engineered bone fabricated by coral, BMSCs and sustainedly released rhBMP-2 by collagen. Methods: Three scaffolds were fabricated: rhBMP-2/coral group, collagen/rhBMP-2/coral group and BMSCs/collagen/rhBMP-2/coral group. Osteogenesis was evaluated after implantation of scaffolds under skin of nude mice 8 weeks later. Results: It showed that osteogenesis in BMSCs/collagen/ rhBMP-2/coral group was more than that in collagen/rhBMP-2/coral group, and osteogenesis in rhBMP-2/coral was least. Conclusion: Collagen may be a suitable sustained release system for rhBMP-2 in animal experiment. And BMSCs may have important effect on enhancing ectopia osteogenesis.
出处
《口腔医学研究》
CAS
CSCD
2004年第6期574-576,共3页
Journal of Oral Science Research
基金
国家"973"项目资助课题 (编号 :G19990 5 43 0 8)
