外消旋聚乳酸/神经生长因子复合导管修复大鼠坐骨神经缺损的量效作用

Dose effect of PDLLA/NGF compound conduit on restoration of rat sciatic nerve defect

目的 探讨外消旋聚乳酸 /神经生长因子 (polyd ,l lacticacid/nervegrowthfactors ,PDLLA/NGF)复合导管对大鼠坐骨神经缺损修复的量效作用。方法 分别以PDLLA导管和含有 40 0 μ、80 0 μNGF的PDLLA/NGF复合导管桥接于大鼠坐骨神经缺损处。术后 3个月用电生理测定、组织学检测等方法观察神经再生情况。结果 在PDLLA/NGF复合导管中 ,NGF含量为 40 0 μ组 ,较含量为 80 0 μ组和PDLLA导管组的坐骨神经再生效果好。结论 PDLLA/NGF复合导管中NGF对坐骨神经缺损的修复存在量效关系 ,适量的NGF能促进神经再生 ,而含量过高反而对神经再生不利。

Objective To investigate the dose effect of poly d, l-lactic acid/nerve growth factor (PDLLA/NGF)compound conduit on restoring rat sciatic nerve defect. Methods The sciatic nerve defect of rats was bridged by PDLLA conduit, PDLLA/NGF compound conduit with 400μ NGF, and PDLLA/NGF compound conduit with 800 μ NGF. The results were evaluated in 3 months following operation by using electrophysiological and histological assessment. Results Nerve regeneration in PDLLA/NGF compound conduit with 400μ NGF was significantly superior to that in the other 2 groups. Conclusion NGF in PDLLA/NGF compound conduit has a dose-effect relationship on the repair of sciatic nerve defect. Suitable dosage of NGF can promote nerve regeneration, while too high dosage can inhibit nerve regeneration.