特异识别已分化PC12细胞的ssDNA适体的结构和功能研究

Structure and function of the ssDNA aptamer specifically recognizing differentiated PC12 cells

目的 :研究特异识别已分化PC12细胞的ssDNA适体的二级结构及截短前后的适体与已分化PC12细胞的结合能力。方法 :利用RNAstructure 3.5软件对截短前后的适体的空间结构进行模建 ,体外合成这些截短适体 ,利用流式细胞术和荧光显微镜研究适体与已分化PC12细胞的结合能力。结果 :单茎环结构是适体与已分化PC12细胞结合的二级结构 ;截短后的适体与已分化PC12细胞的结合能力明显大于与未分化PC12细胞结合能力 ;AP17 38与已分化PC12细胞结合的荧光强度明显大于与未分化PC12细胞结合的荧光强度 ;截短前后的适体均能够特异识别混合物中已分化PC12细胞。结论 :截短后的适体能够保留适体的空间结构 ,而且与已分化PC12细胞的亲合力不会降低。

s Objective: To study the structure and function of the ssDNA aptamer specifically recognizing differentiated PC12 cells. Methods RNA structure 3.5 software was used to simulate the steric structure of the aptamer 17 and its truncated form. The flow cytometry and fluorescent microscopy were used to analyze aptamer binding activity to PC12 cells. Results: The single stem-loop structure was the basic structure of the aptamers specifically binding to differentiated PC12 cells; truncated aptamers with this basic structure could specifically bind to differentiated PC12 cells. No binding to undifferentiated PC12 cells was observed using both flow cytometry and fluorescent microscopy. Aptamer 17 and its truncated form could specifically recognize the differentiated PC12 cells from a mixture of various cells. Conclusion: Truncated aptamers showed the similar basic structure and binding ability to aptamer 17.