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人甲状旁腺素_(1-34)及其聚乙二醇修饰物对鸡血钙含量的影响 被引量：1

Effect of human parathyroid hormones(1-34) and its pegylation on plasma calcium of chicks
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摘要 目的 :通过观察人甲状旁腺素 (hPTH1 3 4 )及其聚乙二醇修饰物对鸡血钙含量的影响判断它们的体内活性。方法 :12日龄的小鸡分别翼静脉注射赋形剂 ,hPTH1 3 4 ,Cys(mPEG50 0 0 MAL) hPTH1 3 4 和hPTH1 3 4 Cys(mPEG50 0 0 MAL) NH2 。 6 0min和 12 0min后翼静脉采血 ,分离血清并测定钙含量。结果 :给药后 6 0min ,hPTH1 3 4 ,Cys(mPEG50 0 0 MAL) hPTH1 3 4 和hPTH1 3 4 Cys(mPEG50 0 0 MAL) NH2 组静脉血清钙含量均明显升高 ;12 0min后 ,Cys(mPEG50 0 0 MAL) hPTH1 3 4 组血清钙含量仍维持升高水平 ,而hPTH1 3 4 和hPTH1 3 4 Cys(mPEG50 0 0 MAL) NH2 组血清钙含量则明显回落。结论 :合成人甲状旁腺素 (hPTH1 3 4 )及其聚乙二醇修饰物可以明显升高小鸡的血清钙水平 ;N端聚乙二醇修饰的hPTH1 3 4 维持血钙升高的时间较长。 Objective: To study the effect of human parathyroid hormones(1-34)(hPTH 1-34) and its pegylation on plasma calcium of chicks. Methods: Chicks(12 days old) were injected via wing veins with hPTH 1-34,Cys(mPEG 5000-MAL)-hPTH 1-34or hPTH 1-34-Cys(mPEG 5000-MAL)-NH 2 at a dose of 10 U/chick, respectively and bled 60 or 120 min later. The plasma calcium levels of serum samples were measured. Results: Sixty minutes after i.v. injection, the serum calcium levels were raised significantly in all three groups. Two hour after i.v. injection it was observed that the raised plasma calcium level was still retained only in the Cys(mPEG 5000-MAL)-hPTH 1-34 group. Conclusion: hPTH 1-34,Cys(mPEG 5000-MAL)-hPTH 1-34 and hPTH 1-34-Cys(mPEG 5000-MAL)-NH 2 could cause the hypercalcemic response of chicks. The effect of Cys(mPEG 5000-MAL)-hPTH 1-34 lasted longer than that of the others.
作者 高亚萍 潘和平 王良友 柳川
机构地区 军事医学科学院基础医学研究所
出处 《军事医学科学院院刊》 CSCD 北大核心 2004年第6期542-543,共2页 Bulletin of the Academy of Military Medical Sciences
关键词 人甲状旁腺激素 聚乙二醇化修饰 生物活性测定 钙 human parathyroid hormones pegylation bioassay calcium
分类号 R96 [医药卫生—药理学]
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参考文献8

  • 1美国和欧洲2002年批准的新药[J].制药原料、中间体及医药生物信息,2003,(11):42-44.
  • 2Veronese FM. Peptide and protein PEGylation: a review of problems and solutions[J].Biomaterials, 2001, 22(5): 405-417.
  • 3Kozlowski A, Harris JM. Improvements in protein PEGylation: pegylated interferons for treatment of hepatitis C[J].J Control Release, 2001, 72(1-3):217-224.
  • 4李力,郑意端.蛋白药物聚乙二醇化技术的研究进展[J].中国临床药理学杂志,2003,19(3):226-229. 被引量：15
  • 5Gonzalez EA,Martin KJ. Coordinate regulation of PTH/PTHrP receptors by PTH and calcitriol in UMR106-01 osteoblast-like cells[J].Kidney Int,1996,50(1):63-70.
  • 6Parsons JA, Reit B, Robinson CJ. A chick bioassay for parathyroid hormone[J].Endocrinology,1973,92(2):454-462.
  • 7王良友,潘和平,柳川,高亚萍.人甲状旁腺素(1-34)的合成与聚乙二醇化修饰[J].中国生化药物杂志,2005,26(2):76-79. 被引量：6
  • 8Kenny AD, Dacke CG. The hypercalcaemic response to parathyroid hormone in Japanese quail[J].J Endocrinol, 1974,62(1):15-23.

二级参考文献25

  • 1Feeney RE. Chemical modification of proteins: comments and perspectives, Int J Pept Protein Res, 1987;29:145.
  • 2Veronese FM. Peptide and protein PEGylation: are view of problems and solutions. Biomaterials, 2001 ;22:405-417.
  • 3Yuji I, Makoto F, Hideyuji S, et al. Biomedical and biotechnological 1 applications of PEG-and PM-modified proteins. Tibtech, 1995; 13:86-91.
  • 4Farruggia B, Nerli B, Nuci HD, et al. Thermal features of the bovine serum albumin unfolding by polyethylene glycols, Int J Biol Macromol, 1999;26:23.
  • 5Fischer A, Hacein-Bey S, Cavazzana-Calvo M, et al. Gene therapy of severe combined immunodeficiencies. Nature Reviews Immunology, 2002;2:615-621.
  • 6Francois J J, Frotier G. Immobilization of L asparaginase into a biocompatible poly(ethylene glycol)albumin hydrogel I: preparation and in vitro characterization. Biotechnol Appl Biochem,1996;23:221-226.
  • 7Sekine T, Kodera Y, Saito T, et al. L asparaginase modified by poly(ethylene glycol) derivative with a comb shaped form anti tumor activity and induction of immune tolerance. Biomed Res, 1995;16:121.
  • 8Kodera Y, Sekine T, Yasukohchi T, et al. Stabilization of L asparaginase modified with comb shaped poly(ethylene glycol) derivatives, in vivo and in vitro.Bioconjugate Chem, 1994;5:283.
  • 9Knusli C, Delgado C, Malik F, et al. Polyethylene glycol(PEG)modification of granulocyte macrophage colony stimulating factor(GM CSF) enhances neutrophilpriming activity but not colony stimulating activity. Br J Hematol, 1992;82:654-663.
  • 10Somack R, Saifer M G, Williams L D. Preparation of longacting superoxide dismutase using high molecular weight polyethylene glycol. Free Radic Res Commun, 1991;12/13:553-562.

共引文献19

同被引文献15

  • 1田玉岭,赵伟,沈玲,刘伟,常家宝,方之勋,殷卫卫,王雷,孙溪宾,杨毅军.聚乙二醇化干扰素α-2a治疗慢性乙型肝炎的临床研究[J].中华肝脏病杂志,2006,14(11):806-810. 被引量：17
  • 2张继娟,吴梧桐.聚乙二醇(PEG-5000)对胰激肽释放酶化学修饰的初步研究[J].药物生物技术,2006,13(6):409-412. 被引量：7
  • 3Sedgwick JB,Jansen KJ,Kennedy JD,et al. Effects of the very late adhesion molecule 4 antagonist EAY103 on human peripheral blood eosinophil vascular cell adhesion molecule- 1 dependent functions [J].J Allergy Clin Immunol,2005,116(4): 812-819.
  • 4Vanderslice P, Biediger RJ,Woodside DG,et al. Development of cell adhesion molecule antagonists as therapeutics for asthma and COPD[J]. Pulm Pharmacol Ther,2004,17( 1 ): 1-10.
  • 5Atsuki F, Tomoko Y,Vaka I,et al.Role of VLA-4 in the development of allergic conjunctivitis in mice[J] .Molecule Vision, 2006,12:310-317.
  • 6David M,Rose.The role of the α 4 integrin-paxillin interaction in regulating leukocyte trafficking[J].Experimental and Molecular Medicine,2006,38(3): 191-195.
  • 7Abraham WM,Gill A,Ahmed A,et al.A small molecule,tightbinding inhibitor alpha(4)beta( 1 ) blocks antigen-induced airway responses and inflammation in experimental asthma in sheep[J] .Am J Respir Crit Care Med,2000,162(2):603-611.
  • 8Gloria CK,Kashmira S,Gloria J,et al.A small molecule very late antigen-4 antagonist can Inhibit ovalbumin-induced lung inflammafion[J].American Journal of Respiratory and Critical Care Medicine,2003,167(10), 1400-1409.
  • 9Dutta AS,Gormley JJ,Coath M,et al.Potent cyclic peptide inhibitors of VLA-4( α 4 β1 integrin)-mediated cell adhesion. Discovery of compounds like cyclo(MePhe-Leu-Asp-Val-D- Arg-D-Arg)(ZD7349) compatible with depot formulation[J].J Pept Sci2000,6(8):398-412.
  • 10Pepinsky RB,Lee W,Comebise M,et al.Design, synthesis, and analysis of a polyethelene glycol-modified(PEGylated) small molecue inhibitor of integrin α4β1 with improved pharmaceuticalproperities[J].Aspetoumals,2005,312(2):742- 750.

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军事医学科学院院刊
2004年 第6期

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