缺血预处理对犬脊髓损伤及热休克蛋白70表达影响的研究

Effect of ischemic pretreatment on expression of heat shock protein 70 and injury of spinal cord in canine

目的 评价缺血预处理对犬脊髓损伤及热休克蛋白 70表达的影响。方法 4 1条杂种犬随机分成假手术组 6只、预处理组 2 1只、对照组 14只。预处理组主动脉阻断 6min后开放 6min ,反复 2次 ,之后阻断 35min ;对照组主动脉阻断 35min。术后进行神经功能评分 ,检测脊髓组织中热休克蛋白 70表达。结果 在再灌注后 6h、2 4h预处理组热休克蛋白 70于胞质和胞核均有表达 ,且强于对照组 ;而且神经功能评分预处理组高于对照组。在再灌注后 7d预处理组神经功能评分无明显改变 ,且仍见热休克蛋白 70表达。结论 缺血预处理可以增加脊髓的缺血耐受 ;热休克蛋白

Objective To evaluate effect of ischemic pretreatment on expression of heat shock protein 70 (HSP70) and injury of spinal cord in canine. Methods Fourty-one canine were divided into three groups : the sham-operative group , the pretreatment group and the control group. In the pretreament group aorta was obstructed for 6 min , and then was opened for 6 min , this procedure was repeated twice , finally arota was obstructed for 35 min. In the control group aorta was obstructed for 35 min .Nervous function were assessed and HSP70 expression were detected in tissue of spinal cord. Results In the pretreatment group , HSP70 expressed in cytoplasm and nucleus at 6 ,24 hour after reperfusion , and intensity of HSP70 expression was stronger than that in the control group ; The score of nervous function in the pretreatment group was higher than that in the control group . On 7 day after reperfusion the score of nervous function in pretreatment group had no obvious variation , and HSP70 expression was still observed. Conclusions Ischemic pretreament can improve ischemic tolerance of spinal cord ; HSP70 expression in cytoplasm and nucleus may play a role in ischemic tolerance.