摘要
目的 研究三氧化二砷、人参皂甙、β榄香烯及环磷酰胺 (CTX )等几种药物 ,在不同浓度时对K5 62细胞株端粒酶活性的调节作用 ,研究上述中西药的作用机制 ,为寻求治疗急性白血病的中西药联合新方法提供参考。方法 用 3组不同浓度的三氧化二砷、人参皂甙、β榄香烯及CTX与人红白血病细胞株K 5 62共同培养 ,分别于作用 2 4、48、72h后收集细胞并采用PCR ELISA法检测K 5 62细胞端粒酶活性。结果 ①人参皂甙、三氧化二砷、β榄香烯及CTX作用后 ,K5 62细胞株端粒酶活性下降 ,下降程度呈浓度时间依赖性 ,在一定浓度及作用时间后端粒酶呈阴性。当三氧化二砷、人参皂甙、β 榄香烯与CTX联合应用时 ,对端粒酶活性的抑制作用增强。②上述药物作用后K 5 62细胞的存活率下降 ,且抑制作用呈浓度、时间依赖性。当三氧化二砷、人参皂甙、β 榄香烯与CTX联合应用时 ,抑制作用增强。 结论 ①三氧化二砷、人参皂甙、β 榄香烯及CTX均能完全抑制K5 62细胞株的端粒酶活性 ,抑制端粒酶活性可能是其抗肿瘤作用的机制之一。当三氧化二砷、人参皂甙、β 榄香烯与CTX联合应用时 ,对端粒酶活性的抑制作用被增强。②上述药物能抑制K 5 62细胞的生长 ,当三氧化二砷、人参皂甙、β 榄香烯与CTX联合应用时 ,抑制作用增强。
Objective To investigate the modulating effect of arsenic trioxide,ginseng saponin,β-elemol and cyclophosphamide(CTX) on telomerase activity in K562 cell line and its mechanism.Methods Human erythroleukemia cell line K562 was cultured with different concentration of arsenic trioxide,ginseng saponin,elemol or combined with CTX for 24,48 and 72 hours.Telomerase activity was detected by the methode of PCR-ELISA.Results ①Telomerase activity of K562 cells decreased after the treatment of arsenic trioxide,ginseng saponin,β-elemol and CTX in a dose- and time-depended manner.The telomerase activity became negative after the treatment with proper dose of the medicine for neccessary time.When the former three drugs were used combined with CTX,the inhibitory effect was enhanced.②The viability of K562 cells decreased after the application of arsenic trioxide,ginseng saponin and β-elemol in a dose- and time-depended manner.The inhibitory effect were enhanced when they were used combined with CTX.Conclusion ①Arsenic trioxide,ginseng saponin,β-elemol,and CTX can completely inhibit telomerase activity in K562 cell line respectively.CTX can enhanced the inhibitory effect of the former three drugs.②Arsenic trioxide,ginseng saponin,β-elemol and CTX can also inhibit the growth of K562 cells.Combined with CTX,the inhibitory effect of the three former drug is strengthened.
出处
《实用癌症杂志》
2004年第5期481-484,共4页
The Practical Journal of Cancer
