薄层扫描法测定肝豆灵片中大黄素含量

Determination of emodin in gandouling tablets by TLC-scanning

目的 建立肝豆灵片中大黄素含量测定方法。方法 薄层扫描法 ,展开剂 :正己烷 乙酸乙酯 甲酸 (30∶10∶0 5 ) ,检测波长4 2 0nm。结果 大黄素在 0 2 0 2～ 1 0 10 μg范围内线性良好 ,相关系数r=0 9986 ,平均回收率为 97 9% ,RSD =1 12 %。结论 本法快捷 ,简便 ,准确 ,可用于肝豆灵片中大黄素的含量测定。

Aim To establish a method for determination of emodin in gandouling tablets.Methods Emoin in gandouling tablets was determinated by TLC-Scanning with a mixture of n-hexane-ethylacetate-formic acid (30 ∶10 ∶0.5)as the developing system.The detection wavelength was 420 nm.Results The calibraction curve was linear in the range of 0.202～1.010 μg,r=0.9986,average recovery rate was 97.9%,RSD=1.12%.Conclusion The method was puick,simple,accurate and suitable for the content determination of emodin in gandouling tablets.