有机锰源络合率的研究

Study on Complexed Mn Percentages in Organic Manganese Sources

利用红外光谱法、凝胶过滤色谱法和阳离子交换平衡法,分析了甘氨酸锰的红外光谱特征、甘氨酸锰和蛋氨酸锰在pH7、0. 1mol·L-1 Tris缓冲溶液中的络合率。结果表明,所合成的甘氨酸锰在红外光谱图上表现出锰络合物的结构特征;在本研究所设定的检测条件下,用凝胶过滤色谱法和阳离子交换平衡法均未检测到甘氨酸锰和蛋氨酸锰在pH7、0 .1mol·L-1 Tris缓冲液中有络合锰的存在。结果提示,由于锰离子形成络合物的能力比较弱,溶液中的络合锰在凝胶过滤和离子交换过程中,可能因络合平衡破坏而在分离过程中发生解离,从而未能检测出络合形态的锰,故不能由此否定氨基酸锰溶液中无络合锰存在。

The structural characteristics of manganese glycine chelate (Mn-Gly) was determined by infrared spectroscopy. The percentages of complexed Mn in Mn-Gly and manganese methionine chelate (Mn-Met) were determined in the solutions of 0.1 mol·L^(-1) Tris buffer (pH 7) by gel filtration chromatography and cation exchanging equilibrium. The results showed that: 1) Mn-Gly chelate had characteristic peaks of complexed Mn; 2) Under the present experimental condition, no complexed Mn was found for Mn-Gly and Mn-Met chelates in pH 7, 0.1 mol·L^(-1) Tris buffer solutions. The results suggest that the reason why complexed Mn was not found in solutions was possibly the complexed Mn dissociated during the processes of gel filtration and cation exchanging, because of the weak binding energy of Mn and the ligands. It can not be concluded that no complexed manganese existed in the solutions of Mn-Gly and Mn-Met according to the results. In addition, the results of this study did not show the situations in vivo, so more studies should be focused on the conditions of the intestine of animals to investigate the absorption characteristics of organic manganese.