可溶性细胞间粘附分子-1与乙型肝炎患者肝细胞损伤和肝纤维化关系的研究

Relation Between Soluble Intercellular Adhesion Molecule-1 Level, Indexes of Liver Function and Liver Fibrosis in Patients with Hepatitis B

【目的】探讨可溶性细胞间粘附分子 1(sICAM 1)在慢性乙型病毒性肝炎 (CHB)、重型乙型病毒性肝炎 (CSH)及肝硬化 (LC)患者血清中的水平及其与肝纤维化的关系。【方法】sICAM 1的检测采用ELISA法 ,并同时采用放免法检测血清透明质酸 (HA)、Ⅲ型前胶原蛋白 (PⅢP)、层粘蛋白 (LN)及Ⅳ型胶原 (CⅣ ) ;以2 0例健康献血员作对照 (NC)。【结果】CHB、CSH、LC患者血清sICAM 1水平均高于对照组 ,血清sICAM 1水平随着患者肝损害的加重而升高 ,并且其升高与LN、CⅣ、PⅢP、HA水平的升高相关。【结论】CHB、CSH、LC患者血清sICAM 1水平升高 ,可反映肝细胞损伤程度及肝纤维化程度。

ObjectiveTo observe the relation between serum soluble intercellular adhesion molecule-1(sICAM-1)and liver fibrosis in chronic hepatitis B(CHB), chronic severe hepatitis B(CSH) and liver cirrhosis(LC).Serum sICAM-1 was detected by enzyme-linked immunosorbent assay( ELISA),meanwhile serum level collagen type Ⅳ( CⅣ),laminin(LN),procollagen peptide(PⅢP),hyaluronic acid(HA) were detected by radioimmunoassay(RIA).Serum sICAM-1, CⅣ,LN,PⅢP,HA levels in CHB,CSH and LC were significantly higher than those in normal control (P<0.01/ P<0.05 ),and increased successively in mild,moderate and severe degrees of CHB,CSH and LC (P<0.01/ P<0.05) and were correlated with degrees of liver damage positively(P<0.01/ P<0.05). Serum sICAM-1 was correlated with serum levels of CⅣ,LN,PⅢP,HA positively(P<0.01/ P<0.05).[Conclusion]Serum sICAM-1 levels of patients with CHB,CSH and LC are useful for the determination of the severity of liver disease and hepatic fibrosis.