慢性低氧及运动训练对大鼠血清一氧化氮(NO)含量及一氧化氮合酶(NOS)活力的影响

Influences of Chronic Hypoxia and Exercise Training on Serum Nitric Oxide(NO) Content and Nitric Oxide Synthase(NOS) Activity in Rats

为观察慢性低氧及训练对大鼠的影响,取健康SD大鼠28只,随机分成4组:(1)常氧对照组(NC),(2)常氧训练组(NT),(3)低氧安静组(HC),(4)低氧训练组(HT)。其中HC、HE两个低氧组每天保证22h生活在模拟4000m高原的低氧舱(氧浓度12 7%),NT、HT两个训练组则每天进行跑台训练1h。28天后,各组均于安静状态下宰杀、取血,测定血清一氧化氮(NO)含量及一氧化氮合酶(NOS)活力。结果可见:28天后,与对照组相比,低氧安静组(HC)和低氧训练组(HT)大鼠血清NO浓度有较显著的降低(P<0 10),而常氧训练组(NT)下降不显著;另一方面,慢性28天后,与常氧对照组(NC)相比,常氧训练组(NT)及低氧对照组(HC)NOS活性虽有上升,但差异不显著;低氧训练组(HT)NOS活性则明显低于NT组及HC组(P<0 05)(P<0 10),这说明低氧及运动两种因素的效应并非是简单的叠加,可能相互抵消。其机制有待进一步研究。

To observe the influence of hypoxia and exercise training on rats , 24 SD rats were randomly divided to four groups : (1) Control at normoxic condition(NC), (2) Training at normoxic condition(NT), (3) Control at hypoxic condition(HC) ,(4)Training at hypoxic condition(HT).The rats of HC and HT were laid in hypoxic cabin(corresponding to an altitude of 4000m,oxygen level 12.7%) for 22h/days,and the ones of HE and OE were trained 1h/day on the treadmill. After 28 day, the rats of four groups were all killed and the blood samples were collected immediately. Then serum NO level and NOS activity were measured and analyzed. The effects showed that the serum NO contents of HC and HT was lower significantly than that of NC group (P<0.10), but those of NT group didn't change significantly when compared with those of NC group. On the other hand, the serum NOS activity of HT group decreased significantly when compared with those of HC and NT group (p<0.05) (P<0.10). As a whole, hypoxia can induce the decrease of he serum NO content, and there is an interaction between chronic hypoxia and exercise training on the NOS activity.