摘要
Background In previous work, we suggested that some 2aminosteroids inhibited proliferation and induced differentiation of both human and murine leukemia cells Here, we reported the actions of another new 2aminosteroid designated as H89712 on human leukemia cells Methods Cell colony counting and MTT assay were used to determine proliferation Cell morphology, histochemical staining, UV detection and cytometry were used to determine differentiation RTPCR was used to detect gene expression. Standard statistical method was used to analyze dataResults H89712 inhibited proliferation of HL60 leukemia cells and the inhibition percentage in MTT assay was 18% at the dose of 108 mol/L and 65% at the dose of 105 mol/L, respectively The inhibition for HL60 in colony assay was 23% at the dose of 108 mol/L and 96% at the dose of 105 mol/L, respectively H89712 also induced HL60 cells toward macrophagelike differentiation It was verified by flow cytometry that the percentage of positive CD14 expression in differentiated HL60 cells was about 9 times higher than that of the control at the dose of 108 mol/L and 20 times higher than that of the control at the dose of 105 mol/L respectively, and this action involved upregulation of MafB gene in HL60 leukemia cells On the other hand, H89712 inhibited proliferation of K562 leukemia cells and the inhibition of K562 leukemia cells in MTT assay was shown by 34% at the dose of 108 mol/L and 88% at the dose of 105 mol/L respectively The inhibition of K562 leukemia cells in colony assay was 53% at the dose of 108 mol/L and 100% at the dose of 105 mol/L respectively H89712 also induced K562 cells toward erythroidlike differentiation and it was verified by flow cytometry that the percentage of positive CD71 expression in differentiated K562 cells was about 9 times higher than that of the control at the dose of 108 mol/L and 16 times higher than that of the control at the dose of 105 mol/L respectively This action was related to upregulation of Egr1 gene in K562 leukemia cellsConclusions Our results showed the important roles played by MafB in macrophage differentiation and Egr1 in erythroid differentiation of human myeloid leukemia cells
Background In previous work, we suggested that some 2aminosteroids inhibited proliferation and induced differentiation of both human and murine leukemia cells Here, we reported the actions of another new 2aminosteroid designated as H89712 on human leukemia cells Methods Cell colony counting and MTT assay were used to determine proliferation Cell morphology, histochemical staining, UV detection and cytometry were used to determine differentiation RTPCR was used to detect gene expression. Standard statistical method was used to analyze dataResults H89712 inhibited proliferation of HL60 leukemia cells and the inhibition percentage in MTT assay was 18% at the dose of 108 mol/L and 65% at the dose of 105 mol/L, respectively The inhibition for HL60 in colony assay was 23% at the dose of 108 mol/L and 96% at the dose of 105 mol/L, respectively H89712 also induced HL60 cells toward macrophagelike differentiation It was verified by flow cytometry that the percentage of positive CD14 expression in differentiated HL60 cells was about 9 times higher than that of the control at the dose of 108 mol/L and 20 times higher than that of the control at the dose of 105 mol/L respectively, and this action involved upregulation of MafB gene in HL60 leukemia cells On the other hand, H89712 inhibited proliferation of K562 leukemia cells and the inhibition of K562 leukemia cells in MTT assay was shown by 34% at the dose of 108 mol/L and 88% at the dose of 105 mol/L respectively The inhibition of K562 leukemia cells in colony assay was 53% at the dose of 108 mol/L and 100% at the dose of 105 mol/L respectively H89712 also induced K562 cells toward erythroidlike differentiation and it was verified by flow cytometry that the percentage of positive CD71 expression in differentiated K562 cells was about 9 times higher than that of the control at the dose of 108 mol/L and 16 times higher than that of the control at the dose of 105 mol/L respectively This action was related to upregulation of Egr1 gene in K562 leukemia cellsConclusions Our results showed the important roles played by MafB in macrophage differentiation and Egr1 in erythroid differentiation of human myeloid leukemia cells
基金
ThisworkwassupportedbytheNationalNaturalScienceFoundationofChina(No 39970316)
