摘要
Background An effective purging technique plays an important role in autologous hematopoietic stem cells transplantation Photodynamic therapy (PDT) provides a novel approach for this purpose This study dealt with the purging effects of disulfodiphthalimidomethyl phthalolcyanine zinc (ZnPcS2P2)based photodynamic therapy (ZnPcPDT) Methods Fluorescence intensity of cell extracts was measured using a fluoresence spectrophotometry The proliferative potency of K562 cells and HL60 cells was detected using MTT colorimetric assay, Typan blue dye exclusion method, colony formation test The proliferative potency of normal hematopoietic cells was evaluated using mixture colonyforming unit (CFUMix), granulocytemacrophage colonyforming unit (CFUGM), and erythrocyte colonyforming unit (CFUE) assays K562 cells were mixed with normal mononuclear cells (MNCs) at ratios of 1∶100 and 1∶1000 for creating the model of simulated remission bone marrow Colony formation test and nestedRTPCR were carried out to detect the residual K562 cells in cell mixture Results After a 5hour incubation with ZnPcS2P2, the content of ZnPcS2P2 in normal MNCs was the lowest value At the same time, the content in K562 cells and HL60 cells was very high Therefore, the time point was selected as the optimal one for irradiating the cell suspensions ZnPcPDT could significantly kill proliferative K562 cells and HL60 cells in a dosedependent manner At the concentration of 10 μg/ml, the inhibitory rate of ZnPcPDT on the colony formation was 100% for K562 cells, 897% for HL60 cells 025 μg/ml ZnPcPDT could completely photoinactivate residual K562 cells in the simulated remission bone marrow Under an identical condition, the inhibitory rates of CFUMix, CFUGM, CFUE were 180%, 186%, and 178% respectively Conclusion ZnPcPDT appears to be a promising approach for bone marrow purging
Background An effective purging technique plays an important role in autologous hematopoietic stem cells transplantation Photodynamic therapy (PDT) provides a novel approach for this purpose This study dealt with the purging effects of disulfodiphthalimidomethyl phthalolcyanine zinc (ZnPcS2P2)based photodynamic therapy (ZnPcPDT) Methods Fluorescence intensity of cell extracts was measured using a fluoresence spectrophotometry The proliferative potency of K562 cells and HL60 cells was detected using MTT colorimetric assay, Typan blue dye exclusion method, colony formation test The proliferative potency of normal hematopoietic cells was evaluated using mixture colonyforming unit (CFUMix), granulocytemacrophage colonyforming unit (CFUGM), and erythrocyte colonyforming unit (CFUE) assays K562 cells were mixed with normal mononuclear cells (MNCs) at ratios of 1∶100 and 1∶1000 for creating the model of simulated remission bone marrow Colony formation test and nestedRTPCR were carried out to detect the residual K562 cells in cell mixture Results After a 5hour incubation with ZnPcS2P2, the content of ZnPcS2P2 in normal MNCs was the lowest value At the same time, the content in K562 cells and HL60 cells was very high Therefore, the time point was selected as the optimal one for irradiating the cell suspensions ZnPcPDT could significantly kill proliferative K562 cells and HL60 cells in a dosedependent manner At the concentration of 10 μg/ml, the inhibitory rate of ZnPcPDT on the colony formation was 100% for K562 cells, 897% for HL60 cells 025 μg/ml ZnPcPDT could completely photoinactivate residual K562 cells in the simulated remission bone marrow Under an identical condition, the inhibitory rates of CFUMix, CFUGM, CFUE were 180%, 186%, and 178% respectively Conclusion ZnPcPDT appears to be a promising approach for bone marrow purging
基金
ThisstudywassupportedbyFujianProvincialScienceFoundation(No 99Z153
2003D10)
