转化生长因子 β1免疫反应阳性物在扩展莫尼茨绦虫体内的分布(英文)

Immunohistochemical distribution of transforming growth factor β1 in cestode Moniezia expansa (Cestoda: Cyclophyllidea)

用免疫组化SABC法研究了转化生长因子 β1免疫反应阳性物在扩展莫尼茨绦虫成熟体节内的分布。结果表明 :转化生长因子免疫反应阳性物广泛分布于扩展莫尼茨绦虫成熟体节内。扩展莫尼茨绦虫成熟体节的组织结构包括被膜与实质两部分 ;实质被肌层分为外部的皮质与内部的髓质 ,在皮质内有节间腺、肌细胞、神经细胞、排泄 (焰 )细胞等 ;髓质内主要是卵 ,包于卵外的生殖管道 ,以及管道间的连结组织。免疫组化染色显示节间腺、被膜、卵及子宫壁呈强阳性着色 ,光密度测定结果分别为 0 2 70 5± 0 0 80 4 ,0 2 4 5 5± 0 0 4 17,0 4 6 38± 0 0 6 0 3,0 0 373± 0 0 6 13。皮质区与髓质区均有弥散阳性反应产物。表明转化生长因子在扩展莫尼茨绦虫体内广泛分布 。

Using immunocytochemical technique, strept-avidin biotin complex(SABC) method and the Computer Image Analysis System (Product of Beihang University, China,CMIAS-007), we studied the distribution of transforming growth factor β1(TGFβ1) immunoreactivity in the cestode Moniezia expansa. The mature proglottides of 5 cestode Moniezia expansa were obtained from faeces of alpacas at the Scientific Research Base of Shanxi Agricultural University, Yuci, Shanxi, China. The results showed that TGFβ1 immunoreactivity was abundant and wide spread in the interproglottidal glands, tegumentary epithelium, eggs and uterus. The value of optical density (OD), were 0.2705±0.0804, 0.2455±0.0417, 0.4638±0.0603, 0.0373±0.0613 respectively. Thus we can get the conclusion that TGFβ1 appeares to be widely distributed in Moniezia expansa and it may play an important role in the growth and development of the worm in the host[