紫外分光光度法测定芸香甙聚乙二醇共沉淀物含量

Determination of Rutin-PEG by Ultraviolet Spectrophotometry

采用紫外分光光度法测定自制芸香甙聚乙二醇共沉淀物的含量,测定波长为 256nm,测定液浓度在3μg/mL^18μg/mL范围内,质量浓度(ρ)与吸收度呈良好的线性关系,r=0. 9983,平均回收率为 99. 83%,RSD=0. 17% (n=6)。该法简便,样液稳定,赋形剂对测定无干扰。

Rutin-PEG was determined by ultraviolet spectrophotometry with wavelength at 256 nm, and the range of concentration was at 3~ 18 μg/mL. The concentration (C) and absorbance (A) was kept at good linearity with r 0.9983, and the average ratio of reclaim 99.83% and RSD 0.17% (n= 6). The method was simple, stable and undisturbed with excipient.