蛋白激酶Cα在肝炎后肝硬化患者肝细胞的表达及其意义

Study on expression of PKCα in hepatocytes of patients with posthepatitic cirrhosis

目的 研究蛋白激酶 (PK)Cα在肝炎后肝硬化患者肝细胞内的表达 ,探讨其在肝细胞再生和肝功能改变中的意义。方法 对 5 0例肝炎后肝硬化患者的肝组织和 3 0例正常的肝组织行PKCα免疫组织化学染色。并对不同肝功能分级患者肝细胞的PKCα表达进行对照性研究。结果 正常肝组织的肝细胞PKCα免疫组织化学染色呈阴性或弱阳性 ,平均吸光度为 0 .0 3 0 1±0 .0 0 79;肝炎后肝硬化患者肝组织的肝细胞呈强阳性 ,平均吸光度为 0 .0 685± 0 .0 0 84,差异有统计学意义 (P <0 .0 1) ,不同肝功能分级的肝炎后肝硬化患者肝细胞的PKCα表达差异有统计学意义 (P <0 .0 5 )。结论 PKCα过度表达可能是肝炎后肝硬化肝细胞增生和肝功能改变重要原因。

Objectives To study the changes of the expression of protein kinase Cα (PKCα) in hepatocytes of patients with posthepatitic cirrhosis.Methods Immunochemical straining was carried out to detect the expression of PKCα in hepatocytes of 50 patients with posthepatitic cirrhosis and 30 normal liver tissues obtained from patients with liver rupture.Results The expression levels of PKCα were significantly lower in normal hepatocytes than those in cirrhosis hepatocytes of patients with posthepatitic cirrhosis ( P < 0.01). The PKCαlevel was higher in the hepatocytes in patients whose liver function was classified as Child A than Child B with the difference being Statistically significant ( P < 0.05). Conclusion The expression of PKCαin hepatocytes of patients with posthepatitic cirrhosis was increased,which can enhance the proliferation of hepatocytes.PKCα signal transduction may be engaged in the liver function by regulating the proliferation of hepatocytes and the mechanism responsible for strong capacity of regeneration cirrhotic liver.