缓释碱性成纤维细胞生长因子微球对膜引导性骨再生的作用

Experimental studies of the sustained release bFGF microspheres for enhancement of guided bone regeneration

目的 评估缓释碱性或纤维细胞生长因子 (bFGF)微球对于膜引导性骨再生 (MG BR )的作用。方法 取 60只成年新西兰大白兔 ,以聚 DL 乳酸 (PDLLA)膜建立兔桡骨MGBR模型 ,根据PDLLA膜管内注入的不同成分将其分为微球组、bFGF组和生理盐水组。术后 2、4、8、12周分别处死动物 ,大体观察、X线摄片、组织学观察和图像分析以及骨生物力学检测。结果 术后2周 ,微球组骨断端已有较多的新生骨形成 ,术后 12周新生骨的改建和重塑基本完成 ,髓腔已基本再通。微球组在 2、4周 ,骨小梁直径和面积的平均值均优于其余两组 (P <0 .0 5 )。8、12周时 ,微球组的骨生物力学指标均显著优于其余两组 (P <0 .0 5 )。结论 bFGF微球通过持续释放有活性的bFGF ,能够明显促进MGBR ,具有良好的临床实用价值。

Objective To evaluate the effect of the sustained release bFGF microspheres on membrane guided bone regeneration (MGBR).Methods Sixty New Zealand white rabbits were divided into control group,free bFGF group and bFGF microspheres group randomly.Animal models of MGBR covered with PDLLA membrane tube in bilaterals radii were estabtlished and treated in different methods.The specimens were collected 2,4,8,12 weeks postoperatively.General observation,X ray examination,histological grading and HE staining,biomechanical measurements were performed on the models of MGBR in different groups.Results Histologically,much new bone trabecula was formed 2 weeks postoperatively,and bone reconstruction and remodeling were almost completed 12 weeks postoperatively in the microspheres group.The histologic image analysis revealed that the values of mean diameter and area of the trabecula in the microspheres group were higher than those in the other two groups ( P < 0.05) 2 weeks and 4 weeks postoperatively.The strength of the newly formed bone in the microspheres group was higher than that in the other groups 8,12 weeks postoperatively.Conclusion bFGF microspheres can significantly promote the new bone formation in the MGBR for its sustained release of activity bFGF.