阿斯匹林联合西米替丁调理感染大鼠蛋白质代谢的观察

Interventional effects of aspirin combined with cimitidine on protein metabolism in septic rats

目的 观察联合使用环氧化酶抑制剂阿斯匹林 (Asp)和组织胺H2受体阻滞剂西米替丁 (Cim)对腹腔感染大鼠全肠外营养 (TPN )时蛋白质分解的调理作用。方法 采用 2 8只成年腹腔感染大鼠 ,随机分成对照组 (TPN组 )和实验组 (Asp +Cim组 )。对照组给予常规TPN ,实验组在TPN溶液中加入注射用赖氨匹林 15mg/kg·d-1和西米替丁注射液每天 80mg/kg体重 ,实验持续 4d。测定实验前后体重、血清总蛋白、白蛋白、尿素氮和肌苷的浓度 ;测定每日氮平衡和尿 3 甲基组氨酸 (3 MH)的排出量 ,计算累积氮平衡和累积尿 3 甲基组氨酸排出量。结果 实验组血清学指标实验前后变化的差值比对照组显著减少 ;累积氮平衡显著优于对照组 (P <0 .0 1) ;累积尿 3 甲基组氨酸排出量比对照组显著减少 (P <0 .0 1)。结论 环氧化酶抑制剂阿斯匹林 (Asp)联合组织胺H2受体阻滞剂西米替丁 (Cim )可以调理腹腔感染大鼠全肠外营养 (TPN)时的蛋白质代谢 ,减少蛋白质丢失。

Objective To investigate the effects of cyclo oxygenase inhibitor aspirin (Asp) combined with histamine H2 receptor antagonistor cimitidine (Cim) on protein metabolism in total parenteral nutrition (TPN) in septic rats.Methods Twenty eight SD rats with abdominal infection were randomly divided into control group (TPN group) and experimental group (Asp+Cim+TPN).The total nutritional admixture (TNA) was infused to every rat in each of the group.Asp 15 mg/kg every day and Cim 80 mg/kg every day were given in TNA in experiment group.The experiment lasted for 4 days.Body weight,total serum protein,serum albumin,blood urinary nitrogen (BUN) and creatine (Cr) were determined at the beginning and the end of the study respectively.Nitrogen balance (NB) was estimated daily and the amount of urinary 3 MH were calculated at the end of this study.Results The variety of serological index was significantly reduced in experimental group as compared with that in control group.Cumulated NB was increased and cumulated 3 MH decreasd significantly in the experimental group.Conclusion Asp combined with Cim may intervene protein metabolism in TPN and reduce protein loss in septic rats.