化学发光免疫分析在HBVM定量检测的临床应用

Clinical Application of Quantitative Determination of Hepatitis B Virus Markers(HBVM) with Electro-Chemiluminescence Immunoassay (ECLIA)

目的:评价电化学发光免疫分析在HBVM定量检测的临床应用价值。方法:应用ELECSYS 2010 型全自动电化学发光免疫分析系统定量检测了HBsAg(8074例)、HBeAg(8036例)、抗-HBc(8042例)、抗- HBe(8036例)、抗-HBs(8074例)和抗-HBc-IgM(628例)对检测结果进行统计分析。结果:HBsAg、HBeAg、 抗-HBc、抗-HBe、抗-HBs和抗-HBc-IgM阳性率分别为10.63%、3.68%、61.38%、36.21%、39.26%和 14.65%;该分析灵敏度高,能检测出微量HBsAg和抗-HBc-IgM,对乙肝病人的病程、疗效、预后起到全程动 态监测作用。结论:电化学发光免疫分析定量检测HBVM对乙型肝炎的早期诊断、病程监测和疗效观察等具 有重要的临床应用价值。

Objective To explore the clinical value of quantitative determination of HBVM with ECLIA for early diagnosis and screening of HBV infections. Methods A large number (over 8,000) of sera specimens from patients with varous diseases were examined with ECLIA for six HBVMs (HBsAg n=8074, HBeAg 8036, HBcAb 8042, HBeAb 8036, HBsAb 8074, HBcAb-IgM 628). Results The positive rate of the respective marker was: HBsAg 10.63%, HBeAg 3.68%, HBcAb 61.38%, HBeAb 36.21%, HBsAb 39.26% and HBcAb-IgM 14.65%. The assay was extremely sensitive for early detection of small amount of HBsAg and HBcAb-IgM (Ten times over that of ELISA), which was very useful for early diagnosis and screening of HBV infections. Conclusion Quantitative determination of HBVM with ECLIA is a simple and highly automated proceduce which can be rapidly done (less than 20 min), with extremaly high sensitivity. It proves to be very useful for early diagnosis of HBV infection and monitoring the disease process as well as screening blood donors.