高压氧对大鼠局灶性脑缺血再灌注时神经元特异性烯醇化酶的影响

Effects of hyperbaric oxygen on neuron specific enolase after cerebral ischemia/reperfusion in rats

目的 研究高压氧 (HBO)对大鼠局灶性脑缺血再灌注 (IR)时 ,大鼠脑梗塞体积 ,脑组织病理改变及神经元特异性烯醇化酶 (NSE)含量的影响。方法 用线栓法制备阻断大脑中动脉 (MCA)的局灶性脑 IR模型。Wistar大鼠 30只 ,随机分为 5组 :正常对照组 (N组 ,n=6 ) ,缺血再灌注 2 h+常压空气组 (R1 组 ,n=6 ) ,缺血再灌注 2 4 h+常压空气组 (R2 组 ,n=6 ) ,缺血再灌注 2 h+HBO组 (H1 组 ,n=6 ) ,缺血再灌注 2 4 h+HBO组 (H2 组 ,n=6 )。R1 、R2 、H1 、H2 组缺血时间均为 2 h。R1 、R2 组暴露于常压空气 ,H1 、H2 组暴露于 2 .5 MPa氧气。病理切片用 HE染色 ,用 Swanson方法测定脑梗塞体积 ,用酶联免疫法测定 NSE含量。结果 H1 、H2 组与 R1 、R2 组相比 ,神经元缺血性损害较轻 ,脑梗塞体积缩小 ,NSE含量明显下降 ,差异有统计学意义。结论 HBO能减轻缺血性脑损害 ,保护脑组织。

Objective To investigate the effects of hyperbaric oxygen (HBO) on the volume of cerebral infarction, cerebral pathological changes and the level of neuron specific enolase (NSE), following cerebral ischemia/reperfusion (IR) in rats. Methods IR was performed by ligation of the middle cerebral artery for 2 h and reperfusion. Thirty Wistar rats were randomly divided into 5 groups: i.e. the normal control group (group N, n =6); the 2 h after reperfusion + normal pressure air group (group R 1, n =6); the 24 h after reperfusion + normal pressure air group (group R 2, n =6); the 2 h after reperfusion + HBO group (group H 1, n =6); the 24 h after reperfusion +HBO group (group H 2, n =6). The duration of ischemia in R 1,R 2,H 1,H 2 groups was all 2 h. The R 1,R 2 groups were exposed to normal air pressure. Both H 1 and H 2 groups were exposed to 2.5 MPa oxygen. The duration of ischemia in R 1,R 2,H 1,H 2 groups was all 2 h. HE dyes were used for pathological sections. The volume of cerebral infarction was determined by Swanson's method. NSE in brain tissue homogenate was determined with enzyme linked immunoassay. Results In the H 1,H 2 groups, neuron ischemia injury was comparatively mild, the volume of cerebral infarction reduced and the level of NSE significantly decreased as compared with those of the R 1 and R 2 groups. WTH Conclusions HBO can reduce brain damage induced by ischemia and protect cerebral tissue.