卡托普利对主动脉缩窄性高血压大鼠主动脉PTEN表达的影响

Effect of Captopril on Expression of PTEN in Aorta of Aortic-induced Hypertensive Rats

为探讨肿瘤抑制因子PTEN在血管紧张素II(AngII)介导的高血压动脉重建中的作用 ,观察了PTEN在高血压主动脉中的表达及血管紧张素转换酶抑制剂卡普托利对其表达的影响。缩窄SD大鼠主动脉复制高血压模型 ,将动物分为对照组 ,高血压组和高血压 +卡托普利组。术后 2 8d ,取主动脉以RT PCR法测定PTENmRNA的表达。免疫组化法检测PTEN蛋白在主动脉的表达及定位。结果显示 ,对照组主动脉PTENmRNA表达显著高于高血压组 ,给予卡托普利的高血压大鼠主动脉PTENmRNA表达显著高于高血压组 ,与对照组相似。高血压大鼠主动脉PTEN免疫染色强度低于卡托普利组和对照组。结果表明 ,PTEN在高血压主动脉的表达减少 ,卡普托利逆转了高血压诱导的PTEN表达减少。提示血管紧张素II和压力可能参与PTEN表达的调控 ,PTEN在高血压诱导的动脉重建过程中发挥作用。

This study inquired about the role of tumor s up pressor PTEN in the arterial remodeling of Ang II induced hypertension. The expr ession of PTEN of aorta was examined in the aortic-constricted hypertensive rat s(hypertension group), in the aortic-constricted hypertensive rats treated with captopril(hypertension and captopril group), and in the rats having undergone s ham operation (control group).At day 28 after surgery, the aortas were collected from the groups. The expression of PTEN mRNA was detected by RT-PCR. The expre ssion and location of PTEN protein were determined by immunohistochemistry. The results showed that the expression of PTEN in aorta of the hypertension group w as significantly lower than that of the hypertension and captopril group, and si milarly lower than that of the control group. The intensity of PTEN-positive i mmunohistochemical production in aorta of the hypertension group was weaker than that of the hypertension and captopril group, and likewise, it was weaker than the control. PTEN-positive immunohistochemical production was located in VSMC of aorta.The findings indicated that the expression of PTEN is reduced in hyper tensive aorta,that the reduced PTEN experession can be reversed by captopril tre atment, that AngII and the increased mechanical strain may participate in regula ting expression of PTEN, and that PTEN may play a role in the arterial remodelin g induced by hypertension.