电化学疗法对S180肉瘤及血管生成的抑制作用与机理分析

Inhibitory Effect of Electrochemotherapy on S180 Tumor Growth and Angiogenesis and the Possible Mechanism

利用电脉冲结合博莱霉素对小鼠S180实体瘤处理后发现 ,肿瘤生长受到了明显抑制 ,肿瘤的抑制率高达 95 .5 % ,电化学处理的小鼠肉瘤表面及内部的肿瘤血管分布明显比对照组少。用电脉冲结合抗肿瘤药物盐酸阿霉素处理小鼠S180肉瘤细胞 ,透射电镜观察发现肿瘤细胞线粒体出现肿胀等变化。结果说明 ,电化学疗法 (Elec trochemotherapy ,ECT)对小鼠S180实体瘤的生长有明显的抑制作用 ,可能原因包括抑制肿瘤血管生成和引起肿瘤细胞超微结构发生改变。

This study evaluated the effects of electric pulses comb in ed with antitumor drugs on S180 tumor cells. It was found that the growth of S18 0 sarcoma was inhibited with a maximum inhibition ratio of 95.5% after the use o f electric pulses in combination with the injection of bleomycin (BLM), and the blood vessels of tumor were obviously fewer than those of the untreated tumor in vivo. The mitochondria of S180 tumor cells were swollen after the use of el ectric pulses in combination with adriamycin. The results showed that electroche motherapy has evident inhibitory effect on the growth of S180 sarcoma and the me chanism may involve the suppression of tumor angiogenesis and changes in the ult rastructures of tumor cells.