类金刚石薄膜的碳相成分对其血小板黏附特性的影响

Effects of Carbon Phase Components on Platelets Adhesion for Diamond Like Carbon(DLC)

对不同工艺和条件制备的 7个类金刚石薄膜 (Diamondlikecarbon ,DLC)试样 ,经X光电子能谱 (XPS)碳相成分分析后 ,分别进行了血小板黏附实验、黏附血小板的形貌观察、分类计数和形态指数计算 ,并通过灰色关联分析 ,研究了碳相成分对血小板黏附量、黏附血小板的形态指数的影响。结果显示 :来自全方位离子注入离子束增强沉积工艺的DLC ,其血小板黏附量和形态指数明显小于等离子体化学气相沉积工艺制备的样品 ;在DLC的 5种碳相成分中 ,DLC碳相与血小板黏附量和形态指数的 (负 )关联度远大于其它碳相成分 ,除此之外只有C H和C O碳相与血小板形态指数的 (正 )关联度较大。表明 :(1)DLC碳相对血小板黏附的影响远较其它碳相成分为大 ,增加DLC碳相的含量是优化DLC血液相容性的关键所在 ;(2 )C H和C O碳相对黏附血小板的变形有促进作用 ,须从工艺上抑制其产生或尽可能降低其含量 ;(3)采用全方位离子注入离子束增强沉积工艺有助于改善DLC的血液相容性。这些结论对设计与改进DLC的制备工艺具有重要的指导意义。

In the present paper, adhesive test, morphology observat io n, classified counting and deformation index calculation of the platelets on sev en DLC samples made by different processes were carried out after XPS analysis, respectively. Then the effects of carbon phase components on amounts and deforma tion index of the platelets adhered to the samples were investigated by using th e analysis of T-type correlation degree in the grey system theory. It has been shown from results that the amounts and the deformation index of the platelets a dhered to the DLC samples made by plasma source ion implantation-ion beam enhan ced deposition (PSII-IBED) are obviously less than those of DLC samples made by plasma CVD. The correlation degrees(negative) between the DLC carbon phase an d the amounts as well as the deformation index of platelets are much more than those of the other four carbon phase components; besides,larger correlation degr ees (positive) only appear between the deformation index of platelets and the C -H carbon phase or C-O carbon phase. It has been indicated that: (1) the effec t of DLC carbon phase on platelets adhesion is much greater than that of the oth er four carbon phase components, the key to improvement in the hemocompatibility of DLC is to increase the DLC carbon phase content; (2)it is necessary to restr ain the form or decrease the C-H carbon phase content and C-O carbon phase con tent so as to control their promotive action on deformation of the platelets adh ered to the surface of DLC; (3)using PSII-IBED process to prepare DLC is helpfu l to improving the hemocompatibility of DLC. These conclusions are essential for designing and improving the deposition process of DLC.