周期性机械牵张对离体成纤维细胞作用的研究进展

Recent Researches on the Responses of Fibroblasts Exposed to Cyclic Mechanical Stretching in vitro

周期性机械牵张对离体成纤维细胞的体外培养有重要的影响。这种影响包括对离体成纤维细胞本身的影响 ,如对这些细胞的增殖、分化、细胞在载体上的排列等方面的影响 ;还包括对离体成纤维细胞某些功能的影响 ,这些功能涉及细胞对胞外基质、细胞生长因子、基质金属蛋白酶的合成与分泌等。通过对上述诸方面的最近研究进行综述 ,认为特定的周期性机械牵张可以对成纤维细胞在植入体内前先进行体外活化 ,使其达到较好的功能状态。

Recently,in vitro dynamical cell-culture has been drawing more an d more attention from researchers in the areas of tissue engineer,and a series of researches have demonstrated that cyclic mechanical stretching has significan t effects on the cell proliferation,differentiation,and on the cell alignment on scaffold, as well as on the synthesis of extracellular matrix,cytokines,an d matrix metalloproteinases (MMPs).By focusing on reviewing the culture of seve ral kinds of fibroblasts in vitro,we learned that these cellular responses ment ioned above induced by cyclic mechanical stretching were tested by many precis el y designed experiments,and assumed that cyclic mechanical stretching,if applie d properly,would contribute significantly to our purpose of constructing more s ophisticated tissue-engineered tendon and ligament.