反相高效液相色谱法测定镇祛平口服液3种主要成分的含量

Content Determination of the 3 Major Components of Zhenquping Oral Liquid with RP-HPLC

目的 采用反相高效液相色谱法测定镇祛平口服液中盐酸曲普利定 (C)、愈创甘油醚 (G)和氢溴酸右美沙芬 (D) 3种主要成分的含量。方法 色谱柱 :YWGC1 8柱 (15 0mm× 5mm ,10 μm) ;流动相 :770mL甲醇 2 3 0mL重蒸纯化水 (含 5mmol·L 1 庚烷磺酸钠 ,用磷酸调pH值为 3 0 ) ;在波长 2 62nm处进行检测。结果 C在 8 5 94～ 77 786μg·mL 1 ,G在 188 8～ 1698 8μg·mL 1 ,D在 41 0 8～ 3 69 72 μg·mL 1 浓度范围内线性关系良好。C的回收率 =99 9% ,RSD =0 80 % ;G的回收率 =10 1 6% ,RSD为 0 69% ;D的回收率 =10 0 5 % ,RSD为 0 68%。结论 该法简便、快速、准确 ,可用于镇祛平口服液中 3种主要成分的含量测定。

Objective To set up a RP-HLPC method for the content determination of triprolidine hydrochloride(C), guaifenesin(G) and dext romethorphan hydrobromide(D)in zhenquping oral liquid. Methods A YWG ODS C 18 column(150 mm×5.0 mm, 10 μm) was used as the stationary phase. The mobile phase consisted of 770 mL meth anol and 230 mL water(containing 5 mmol·L -1 sodium heptane sulfonate,th e pH value being adjusted to 3.0 with phosphoric acid ).The detection wavelengt h was 262 nm. Results Fairly good linear relationships were shown whe n the concentrations of C,G and D were in the ranges of 8.594-77.786 μg·mL -1, 188.8-1 698.8 μg·mL -1,and 41.08-369.72 μg·mL -1 res pectively. The average recovery rates of C,G and D were 99.9%,RSD=0.80%;10 1.6%,RSD=0.69% and 100.5%,RSD=0.68%,respectively. Conclusion The method was shown to be simple, convenien t, rapid and accurate. It may therefore be used in the content determination of the 3 major components in zhengquping oral liquid.