氯化锂对染铅大鼠神经行为的影响

Effects of Lithium Chloride on Neurobehavior of Lead-exposed Rat

目的探讨不同浓度氯化锂对染铅大鼠神经行为的影响。方法将大鼠随机分为对照组、染铅组、四个氯化锂组和四个铅+氯化锂组,分别给予普通饲料和含氯化锂(3,30,300和3000mg/kg)的饲料喂养,染铅组和四个铅+氯化锂组饮用体积分数0.2%PbAc2的单蒸水,喂养60d,通过测量大鼠体重和Y-迷宫实验,观察各组大鼠之间学习记忆能力的差别;采用黄递酶(NADPH-d)组织化学染色方法观察各组大鼠海马一氧化氮合酶(NOS)阳性细胞数的差别。结果氯化锂(3,30mg/kg)组大鼠体重增长多于对照组,Y-迷宫实验学会次数均少于对照组,差异均有显著性(P<0.05);氯化锂(300、3000mg/kg)组、染铅组体重增长少于对照组,Y-迷宫实验学会次数均多于对照组,差异均有显著性(P<0.05);与染铅组相比,铅+氯化锂(3,30,300mg/kg)组大鼠体重增量增加,Y-迷宫实验学会次数减少,差异有显著性(P<0.05)。NADPH-d组织化学染色结果示:氯化锂(3,30mg/kg)组大鼠海马一氧化氮合酶(NOS)阳性细胞数明显多于对照组(P<0.05);氯化锂(300、3000mg/kg)组、染铅组则少于正常大鼠(P<0.05);铅+氯化锂(3,30,300,3000mg/kg)组大鼠NOS阳性细胞数均多于染铅组(P<0.05)。结论较低浓度的氯化锂能促进正常大鼠学习记忆能力的提高,而较高浓度的锂则可能损害大鼠的学习记忆能力;

ObjectiveTo investigate the effects of lithium on the neurobehavioral development and function of lead-exposed rat. Methods Wistar rats were randomly divided into control group , lead group, four lithium chloride(LiCl)groups and four lead + LiCl groups. Four LiCl groups and four lead + LiCl groups were feed with food containing 3, 30, 300,3000mg/kg LiCl respectively. lead + LiCl groups and lead group were given distilled water containing 0.2% PbAc2. By means of measuring rat body weight and Y-maze testing, we observed the difference of the body weight gains and the ability of learning and memo-ry of different groups. We compared the NOS positive neurons of CA1,CA3 and dentate gyrus of hip-pocampus by means of NADPH-diaphorased(NADPH-d) histochemistry. Rusults Results of body weight gain and Y-maze: LiCl(3, 30mg/kg)groups were better than Control group (p<0.05). Resultsof LiCl(300, 3000mg/kg)?Lead groups were weaker than Control group(p<0.05);Results of NADPH-d histochemistry: the number of NOS positive neurons of LiCl(3,30mg/kg)groups were higher than that of Control group(p<0.05);the number of NOS positive neurons of LiCl(300,3000mg/kg)?Lead groups were lower than that of Control group(p<0.05);the number of NOS positive neurons of Lead+LiCl(3, 30, 300, 3000mg/kg)groups were higher than that of Lead group(p<0.05)?ConclusionLow dose of lithium could resist the lesion of nervous system induced by lead obviously.