薄层扫描法测定注射用硫酸庆大霉素中3组分的含量

Content Determination of Three Components in Gentamicin with Scan-TLC

目的 :建立注射用硫酸庆大霉素中3组分含量的快速测定方法。方法 :采用薄层扫描法 ,以甲醇 -氯仿 -28 %氨水 (1∶1∶1)为展开剂 ,新配制的茚三酮的乙醇 -冰醋酸溶液为显色剂 ,测定波长为500nm。结果 :注射用硫酸庆大霉素中3组分的点样量均在2μg～12μg 范围内线性关系良好 (r1=0. 9993、r2=0. 9996、r1a=0 .9991)。结论 :薄层扫描法可作为注射用硫酸庆大霉素中3组分含量的快速测定方法。

OBJECTIVE:To establish a rapid determination method for gentaminin active components.METHODS:Scan-TLC procedure was applied.A mixture of methanol-chloroform-28%ammonium hydroxide(1∶1∶1)was taken as developing reagent and ninhydrin in a mixtrue solvent as reveal reagent.The detecting wavelength was500nm.RESULTS:The amounts of three components in gentamincin per spot were linear in the range of2μg～12μg(r 1 =0.9993、r 2 =0.9996、r 1a =0.9991).CONCLUSION:This scan-TLC technique is recommendable for determination of gentamincin components.