摘要
目的 :研究黄柏与甘草及桑寄生共煎对小檗碱提取率的影响。方法 :采用高效液相色谱法对黄柏单煎和与甘草及桑寄生共煎液中小檗碱提取量进行测定 ;色谱柱为HypersilBDSC18,流动相为乙腈 -33mmol/L磷酸二氢钾 -三乙胺 (20∶72∶0. 1) ,检测波长为345nm ,柱温为25℃ ,流速为1. 0ml/min。结果 :黄柏与甘草及桑寄生共煎使小檗碱的提出率明显降低。结论 :对含有上述药物的制剂在制定提取工艺时 ,将黄柏单独煎煮提取较好。
OBJECTIVE:To study the infuence of mixed decoction of Cortex Phellodendri,Radix Glycyrrhizae and Ramuˉlus Loranthi on extraction rate of berberine.METHODS:The extraction amount from Cortex Phellodendri,Radix Glycyrrhizae and Ramulus Loranthi mixed decoction was determined by HPLC.The HPLC conditions were:Hypersil BDS C 18 column;mobile phase,acetonitrile-33mmol/L KH 2 PO 4 -triethylamine(20∶72∶0.1);detecting wavelength,345nm;column temperature25℃;flow fate1.0ml/min.RESULTS:Decoction of Cortex Phellodendri,Radix Glycyrrhizae and Ramulus Loranthi in combiˉnation obviously decreased the extraction rate of berberine.CONCLUSION:Cortex Phellodendri should be extracted separately in formulating extract technic for preparations containing above-mentioned herbs.
出处
《中国药房》
CAS
CSCD
北大核心
2005年第3期235-236,共2页
China Pharmacy
关键词
黄柏
甘草
桑寄生
共煎
小檗碱
提取率
Cortex Phellodendri
Radix glycyrrhizae
Ramulus loranthi
Mixed decoction
Berberine
Extraction rate
