摘要
目的 探讨辛伐他汀对球囊损伤颈动脉后大鼠血管重塑及血管外膜转化生长因子 β1(TGF β1)表达的影响。方法 将雄性SD大鼠随机分为单纯球囊损伤组 (A组 ,n =18)和球囊损伤 +辛伐他汀干预组 (B组 ,n =18)。A组只进行球囊损伤 ,B组在球囊损伤前 1周至术后 14d应用辛伐他汀。分别于术后 2、7、14d取颈总动脉 ,应用计算机图像分析系统观察血管管腔面积 (LA)、内膜面积 (IA)、内弹力板环绕面积 (IEMA)及血管总面积 (TA)的变化 ,采用免疫组织化学法检测血管外膜TGF β1的表达。结果 球囊损伤后 14d ,B组的LA、IEMA、TA明显扩大 ,IA则减小 ;B组各时间点血管外膜TGF β1的表达明显低于A组 (均P <0 .0 1)。结论 辛伐他汀可以改善球囊损伤后的血管重塑 ,这种作用可能是通过降低血管外膜TGF β1的表达实现的。
Objective To investigate the effects of simvastatin on vascular remodeling and transforming growth factor-β1 (TGF、β1) expression in vascular adventitia in carotid of rats with balloon injury.Methods The male SD rats were randomly divided into ballon injury group (group A,n=18) and the ballon injury group adding simvastatin intervention (group B,n=18). The rats were killed and the carotids were removed at 2,7,14 day after balloon injury respectively.Through image analysis system the change of lumen area(LA),intimal area(IA),intermal elastic lamina area (IEMA) and total vessel area (TA) were observed. We also observed the expression of TGF、β1 in vascular adventitia by the method of routine immunohistochemical staining.Results Compared to group A,IEMA,LA and TA of group B were expanded apparently and IA decreased significantly.The expression of TGF、β1 in group B was much lower than that in group A at anytime(all P<0.01). Conclusions Simvastatin may ameliorate vascular negative remodeling and prevent injury-induced reduction by decreasing the conent of TGF、β1 in vascular adventitia.
出处
《现代医学》
2004年第3期152-155,共4页
Modern Medical Journal
