新疆肝病患者HDV、HBV感染的免疫组化研究

Immunohistochemical Study of HDV and HBV Antigens in Liver Tissue from Xinjiang Patients with Hepatic Diseases

作者以直接酶标法及过氧化物酶-抗过氧化物酶法(PAP法)对145例新疆各类肝病患者的肝组织进行HDV、HBV感染的免疫组化研究,各类标本中,HBsAg及HBcAg的合计检出率为39.3％,HDAg在HBV血清学标记阳性患者肝组织中的检出率为3.5％。胞浆型HBcAg表达多见于肝组织病变明显区域,HDAg在肝炎后肝硬化组的检出率明显高于HBV感染非肝硬化组(P<0.025),提示肝炎后肝硬化与HDV感染有关。

145 liver samples from Xinjiang patients with various liver diseases have been detected for HBsAg, HBcAg and HDAg, using direct immunoperoxidase staining and PAP method. The total prevalence of HBsAg and HBcAg is 39.3％. The prevalence of HDAg in the HBV marks positive samples is 3.5％, while in the group of cirrhosis is 13.3％. The liver damage is related to the cytoplasmic present of HBcAg. The results support the assumption that HDV and HBV super infection promotes hepatitis B progressing to cirrhosis.