摘要
由栓皮栎 1个月生的实生苗上采集幼叶和茎段做为外植体诱导胚性愈伤组织。结果表明,NAA和BA结合使用(茎段和带叶柄的叶片: 1. 0~2. 0mg·L-1 NAA+0. 5mg·L-1BA;不带叶柄的叶片: 2. 0~4. 0mg·L-1NAA+1. 0mg·L-1BA)诱导的愈伤组织质量好,胚性愈伤组织较多,增殖能力强。2, 4 D和KT结合使用 (最适配比为: 1. 0mg·L-1 2, 4 D+1. 0mg·L-1 KT),虽然诱导时间短,诱导率高,但是愈伤组织质量差,多呈水煮样组织,胚性愈伤组织少并且增殖能力差。最优的增殖培养体系激素组合为 0. 1mg·L-1 NAA+0. 1mg·L-1BA。
Internodal and leaf segments from one month old Quercus variabilis seedlings were used as explant source to induce embryogenic callis.The results showed that callus induced initially on media conmbined application of NAA and BA(superior concentration: Internodal and leaves with leafstalks best induced in 1.0~2.0 mg·L^(-1)NAA and 0.5 mg·L^(-1) BA; leaves without leafstalks in 2.0~4.0 mg·L^(-1) NAA and 1.0 mg·L^(-1) BA ) have more embryogenic callis and higher proliferation capacity. But have less embryogenic callis and worse proliferation capacity on medium containing 2,4-D and KT(superior concentration:1.0 mg·L^(-1) 2,4-D and 1.0 mg·L^(-1) KT), although they were induced easily and quickly. The best proliferation lines selected was combined with 0.1 mg·L^(-1) BA and 0.1 mg·L^(-1) NAA in MS medium.
出处
《西北林学院学报》
CSCD
北大核心
2005年第1期74-77,共4页
Journal of Northwest Forestry University
基金
陕西省自然科学基金项目(2002C103)
关键词
栓皮栎
胚性愈伤组织
增殖体系
Quercus varibilis
embryogenic callus
proliferation lines
